Ferrioxamine Siderophores Detected amongst Iron Binding Ligands Produced during the Remineralization of Marine Particles
Ferrioxamine Siderophores Detected amongst Iron Binding Ligands Produced during the Remineralization of Marine Particles
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在海洋颗粒再矿化过程中产生的铁结合配体中检测到铁胺铁载体
DOI：
10.3389/fmars.2016.00172
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发表时间：
2016
影响因子：
3.7
通讯作者：
S. Sander
中科院分区：
文献类型：
--
作者：
I. Velasquez;Enitan Ibisanmi;E. W. Maas;P. Boyd;S. Nodder;S. Sander
海洋颗粒物的微生物降解是铁等营养物质矿化的重要过程。作为GEOTRACES过程研究（FeCycle II）的一部分，我们进行了孵化实验，从30和100米深度的海洋颗粒在两个站在南春在新西兰北岛东部的亚热带沃茨。使用原位泵收集的颗粒，并主要包括悬浮和缓慢下沉的人口沿着与相关的附着异养细菌。在用活细菌处理中，观察到Fe结合配体的浓度增加，平均稳定常数为logKFeL，站点1的Fe 3 + = 21.11±0.37，站点2的Fe 3 += 20.89±0.25。配体的释放速率在2.54和11.8 pmol L-1 d-1（计算环境海水颗粒浓度）之间变化，与两项南大洋次表层研究中发现的亚极地和极地沃茨约110 m深处的结果相似。溶解铁（DFe）的释放速率在0.33和2.09 pmol Fe L-1 d-1之间，柱积分（30 - 100 m）通量分别为107和58 nmol Fe m-2 d-1。假设1号站和2号站采样时混合层DFe存量分别为~48 µmol m-2和~4 µmol m-2，则DFe停留时间分别为1.2年和0.18年（假设颗粒矿化是混合层中铁的唯一来源）。计算的DFe释放速率与先前提到的南大洋水团研究中发现的速率相当。发现产生Fe结合配体的细菌（CAS阳性）丰度在7至8天的整个实验期间增加。首次使用化学分析和LC-ESI-MS对铁氧胺型铁载体（包括众所周知的铁氧胺B和G）进行了定量。我们的亚热带研究证实了来自南大洋的先前报告，即颗粒矿化是DFe和配体的重要来源，并通过揭示铁载体可能是颗粒物释放到地下沃茨中的配体的重要组成部分，增加了前所未有的细节。商业化。
The microbial degradation of marine particles is an important process in the remineralization of nutrients including iron. As part of the GEOTRACES process study (FeCycle II), we conducted incubation experiments with marine particles obtained from 30 and 100 m depth at two stations during austral spring in the subtropical waters east of the North Island of New Zealand. The particles were collected using in-situ pumps, and comprised mainly of suspended and slow sinking populations along with associated attached heterotrophic bacteria. In treatments with live bacteria, increasing concentrations of Fe binding ligands were observed with an average stability constant of logKFeL,Fe3+ = 21.11±0.37 for station 1 and 20.89±0.25 for station 2. The ligand release rates varied between 2.54 and 11.8 pmol L-1 d-1 (calculated for ambient seawater particle concentration) and were similar to those found in two Southern Ocean subsurface studies from ~110 m depths in subpolar and polar waters. Dissolved iron (DFe) was released at a rate between 0.33 and 2.09 pmol Fe L-1 d-1 with a column integrated (30 -100 m) flux of 107 and 58 nmol Fe m-2 day-1 at station 1 and 2, respectively. Given a mixed layer DFe inventory of ~48 µmol m-2 and ~4 µmol m-2 at the time of sampling for station 1 and 2, this will therefore result in a DFe residence time of 1.2 and 0.18 years, assuming particle remineralization was the only source of iron in the mixed layer. The DFe release rates calculated were comparable to those found in the previously mentioned study of Southern Ocean water masses. Fe-binding ligand producing bacteria (CAS positive) abundance was found to increase throughout the duration of the experiment of 7 to 8 days. For the first time ferrioxamine type siderophores, including the well-known ferrioxamine B and G, have been quantified using chemical assays and LC-ESI-MS. Our subtropical study corroborates prior reports from the Southern Ocean of particle remineralization being an important source of DFe and ligands, and adds unprecedented detail by revealing that siderophores are probably an important component of the ligands released into subsurface waters during particle remineralisation.
影响因子：
2.9
作者：
SCHWYN, B;NEILANDS, JB
通讯作者：
NEILANDS, JB