Quantitative trait loci approach to the genetics of sleep in recombinant inbred mice
Quantitative trait loci approach to the genetics of sleep in recombinant inbred mice
复制标题
重组近交小鼠睡眠遗传学的数量性状位点方法
作者：
Tafti;Chollet;Valatx;Franken
睡眠是一种复杂的性状，受许多基因、环境以及基因-环境相互作用的影响。在睡眠遗传学的不同方法中，数量性状（QTL）分析具有能够检测影响睡眠的不同数量方面的沿着主基因、次要和/或修饰基因的优点。我们已经使用了两套不同的重组近交系（RI）的QTL分析，并寻求确认在8个组织相容性同源株的几个定位。确定了几个影响警戒状态数量的QTL。在第一个RI系列（7个菌株）中，唯一确定的QTL是影响异相睡眠（PS）的QTL，而在第二个RI系列（25个菌株）中的分析显示了影响PS、慢波睡眠和总睡眠的QTL。其中，第5号染色体上的一个QTL与所有的警觉状态相关，这表明存在一个影响睡眠量的基本方面的主基因。在已鉴定的QTL周围搜索候选基因，发现了几个与睡眠调节有关的免疫相关基因。携带影响这些候选基因的功能丧失和/或功能获得突变的转基因动物应证实这些发现。
Sleep is a complex trait controlled by many genes, the environment, and probably by gene–environment interactions. Among different approaches to the genetics of sleep, analysis of quantitative traits (QTL) has the advantage of being able to detect, along with major genes, minor and/or modifier genes influencing different quantitative aspects of sleep. We have used QTL analysis in two different sets of recombinant inbred (RI) strains and sought for confirmation of several localizations in eight histocompatibility congenic strains. Several QTLs were identified which influenced the amount of vigilance states. In a first RI series (seven strains) the only QTLs identified were those affecting paradoxical sleep (PS), whereas analysis in a second RI series (25 strains) revealed QTLs influencing PS, slow‐wave sleep, and total sleep. Among these, a single QTL on chromosome 5 was associated with all vigilance states, suggesting the presence of a major gene influencing a basic aspect of sleep amount. Search for candidate genes around the identified QTLs indicated several immune related genes that have been implicated in sleep regulation. Transgenic animals carrying loss‐of‐function and/or gain‐of‐function mutations affecting these candidate genes should confirm these findings.
BXD 重组近交小鼠皮质酮对乙醇反应的遗传分析。
影响因子：
1.9
作者：
Roberts,AJ;Phillips,TJ;Belknap,JK;Finn,DA;Keith,LD
通讯作者：
Keith,LD
小鼠癫痫基因图谱。
DOI：
10.1126/science.1871601
发表时间：
1991
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rise,ML;Frankel,WN;Coffin,JM;Seyfried,TN
通讯作者：
Seyfried,TN