Comprehensive CircRNA Profiling and Selection of Key CircRNAs Reveal the Potential Regulatory Roles of CircRNAs throughout Ovarian Development and Maturation in Cynoglossus semilaevis.
Comprehensive CircRNA Profiling and Selection of Key CircRNAs Reveal the Potential Regulatory Roles of CircRNAs throughout Ovarian Development and Maturation in Cynoglossus semilaevis.
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全面的 circRNA 分析和关键 circRNA 的选择揭示了 circRNA 在半滑舌鱼卵巢发育和成熟过程中的潜在调节作用
DOI：
10.3390/biology10090830
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发表时间：
2021-08-26
期刊：
影响因子：
4.2
通讯作者：
Zhang D
中科院分区：
文献类型：
--
作者：
Li J;Shi B;Wang C;Shao C;Liu X;Zhang D
环状RNA：作为参与基因调控的分子，已成为非编码RNA领域新的研究热点。环状rna表现出组织或发育阶段特异性的表达模式，并能影响其亲本基因的表达水平。最近的研究已经记录了环状rna在人类和动物的生长、发育、繁殖和健康中的潜在生物学作用。舌鳎（Cynoglossus semilaevis）是一种海洋比目鱼，是中国重要的养殖物种。近年来，由于野生资源的减少，舌鳎的商业养殖得到了发展。繁殖受脑-垂体-性腺-肝轴调控，限制了人工舌底培养的发展。然而，环状rna在舌底卵巢发育和成熟中的作用尚未报道。环状rna潜在功能的鉴定为理解卵母细胞生长和成熟调控的遗传机制提供了基础，这将有助于提高舌底繁殖的效率。此外，我们的发现扩展了对一种新型内源性RNA参与调节舌底卵巢发育和成熟的认识。环状rna是一种新型的内源性非编码小rna，参与多种生物过程的调控。然而，人们对环状rna在鱼类卵巢发育和成熟中的作用知之甚少。我们的研究首次提供了在卵巢发育的三个阶段舌底组织中环状rna的类型和相对丰度的全基因组概述。我们在大脑中检测到6790个环状rna，在脑垂体中检测到5712个，在卵巢中检测到4937个，在肝脏中检测到4160个。一些circrna表现出组织特异性表达，qRT-PCR在很大程度上证实了6种差异表达（DE） circrna。对DE mrna进行基因本体和KEGG通路分析。一些DE circRNA亲本基因与关键信号通路的生物学过程密切相关，可能在卵巢发育和成熟中发挥重要作用。我们发现所选择的环状rna参与了10种途径。RNase R酶切实验和Sanger测序证实circRNA具有环状结构，具有RNase R抗性。qRT-PCR结果在很大程度上证实了RNA-seq数据中circRNA表达模式的差异。这些发现表明，环状rna广泛存在于舌底的生产相关组织中，在卵巢发育和成熟中具有潜在的重要调节作用。
CircRNAs: as molecules involved in gene regulation, have become a new research hotspot in the non-coding RNA field. CircRNAs show tissue- or developmental stage-specific patterns of expression and can influence the expression levels of their parental genes. Recent studies have documented the potential biological roles of circRNAs in the growth, development, reproduction and health of humans and animals. Tongue sole (Cynoglossus semilaevis) is a marine flatfish that is an economically important farmed species in China. The commercial aquaculture of tongue sole has developed in the last few years because wild resources have decreased. Reproduction is regulated by brain-pituitary-gonad-liver axis which limits the development of artificial tongue sole culture. However, the roles of circRNAs in the ovarian development and maturation of tongue sole has never been reported. The identification of the potential functions of circRNAs provides a foundation for understanding the genetic mechanisms that regulate oocyte growth and maturation, which will allow the efficiency of tongue sole reproduction to be improved. Moreover, our findings extend the knowledge about a new type of endogenous RNA involved in regulating the ovarian development and maturation of tongue sole. CircRNAs are novel endogenous non-coding small RNAs involved in the regulation of multiple biological processes. However, little is known regarding circRNAs in ovarian development and maturation of fish. Our study, for the first time, provides the genome-wide overview of the types and relative abundances of circRNAs in tongue sole tissues during three ovarian developmental stages. We detected 6790 circRNAs in the brain, 5712 in the pituitary gland, 4937 in the ovary and 4160 in the liver. Some circRNAs exhibit tissue-specific expression, and qRT-PCR largely confirmed 6 differentially expressed (DE) circRNAs. Gene Ontology and KEGG pathway analyses of DE mRNAs were performed. Some DE circRNA parental genes were closely associated with biological processes in key signalling pathways and may play essential roles in ovarian development and maturation. We found that the selected circRNAs were involved in 10 pathways. RNase R digestion experiment and Sanger sequencing verified that the circRNA had a ring structure and was RNase R resistant. qRT-PCR results largely confirmed differential circRNA expression patterns from the RNA-seq data. These findings indicate that circRNAs are widespread in terms of present in production-related tissues of tongue sole with potentially important regulatory roles in ovarian development and maturation.
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影响因子：
--
作者：
Liu, Zhilin;Rudd, Michael D.;Richards, JoAnne S.
通讯作者：
Richards, JoAnne S.
人睾丸来源的环状 RNA 的鉴定和表征及其在精浆中的存在
影响因子：
4.6
作者：
Dong WW;Li HM;Qing XR;Huang DH;Li HG
通讯作者：
Li HG
circFGFR4通过结合miR-107解除其对Wnt3a的抑制促进成肌细胞分化
DOI：
10.1016/j.omtn.2018.02.012
发表时间：
2018-06-01
期刊：
Molecular therapy. Nucleic acids
影响因子：
--
作者：
Li H;Wei X;Yang J;Dong D;Hao D;Huang Y;Lan X;Plath M;Lei C;Ma Y;Lin F;Bai Y;Chen H
通讯作者：
Chen H
影响因子：
--
作者：
Fu L;Chen Q;Yao T;Li T;Ying S;Hu Y;Guo J
通讯作者：
Guo J
影响因子：
4.5
作者：
Liu, Bo;Yuan, Rui;Gong, Chengliang
通讯作者：
Gong, Chengliang