Identification and characterization of human testis derived circular RNAs and their existence in seminal plasma.
Identification and characterization of human testis derived circular RNAs and their existence in seminal plasma.
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人睾丸来源的环状 RNA 的鉴定和表征及其在精浆中的存在
DOI：
10.1038/srep39080
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发表时间：
2016-12-13
影响因子：
4.6
通讯作者：
Li HG
中科院分区：
文献类型：
--
作者：
Dong WW;Li HM;Qing XR;Huang DH;Li HG
与其他非编码RNA一样，环状RNA（circRNA）已成为基因调控中感兴趣的新分子。虽然它们已经在一些物种和组织中被探索，但circRNA在人类生殖系统中的表达和功能仍然未知。在这里，我们使用高通量测序揭示了人类睾丸组织中circRNA的表达谱。还研究了精浆中这些睾丸来源的circRNA的构象，旨在为睾丸活检提供非侵入性液体活检替代物。我们预测人类睾丸中有超过15，000个circRNA，其中大多数（10，792; 67%）是新的。在所有5，928个circRNA形成基因中，我们首次报道了1，017个产生circRNA。有趣的是，这些基因大多与精子发生、精子运动、受精等有关。本文还对人类睾丸中circRNA的序列特征、染色体定位、选择性剪接等特征进行了探讨。此外，我们发现这些睾丸来源的circRNA可以在精浆中稳定地检测到。它们大多数可能与精浆中的蛋白质结合，在室温下非常稳定。我们的工作为进一步阐明circRNA在精子发生中的调控机制和开发circRNA作为男性不育疾病的新型非侵入性生物标志物奠定了基础。
Circular RNAs (circRNAs) have emerged as novel molecules of interest in gene regulation as other noncoding RNAs. Though they have been explored in some species and tissues, the expression and functions of circRNAs in human reproductive systems remain unknown. Here we revealed the expression profiles of circRNAs in human testis tissue using high-throughput sequencing. The conformation of these testis-derived circRNAs in seminal plasma was also investigated, aiming to provide a non-invasive liquid biopsy surrogate for testicular biopsy. We predicted >15,000 circRNAs in human testis, with most of them (10,792; 67%) new. In all the 5,928 circRNA forming genes, 1,017 are first reported by us to generate circRNAs. Interestingly, these genes are mostly related to spermatogenesis, sperm motility, fertilization, etc. The sequence feature, chromosome location, alternative splicing and other characteristics of the circRNAs in human testis were also explored. Moreover, we found that these testis-derived circRNAs could be stably detected in seminal plasma. Most of them were probably bound with proteins in seminal plasma and were very stable at room temperature. Our work has laid the foundations to decipher regulation mechanisms of circRNAs in spermatogenesis and to develop circRNAs as novel noninvasive biomarkers for male infertile diseases.
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影响因子：
4.5
作者：
Burd CE;Jeck WR;Liu Y;Sanoff HK;Wang Z;Sharpless NE
通讯作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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