Rapid and simple determination of homovanillic acid in plasma using high performance liquid chromatography with electrochemical detection.
Rapid and simple determination of homovanillic acid in plasma using high performance liquid chromatography with electrochemical detection.
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使用高效液相色谱和电化学检测快速、简单地测定血浆中的高香草酸。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
M. Linnoila
中科院分区：
文献类型：
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作者：
W. Chang;M. Scheinin;R. Burns;M. Linnoila
本文介绍了一种快速、可靠的测定血浆中高香草酸(HVA)的方法。蛋白质经高氯酸沉淀后，直接用反相柱(C8)进行高效液相色谱分离，然后基于电化学检测进行定量。该方法的灵敏度为0.5pmol/次进样。检测器的响应从检测极限到至少0.5nmol/进样都是线性的。在50-150pmol/ml血浆浓度范围内，批内变异系数为2.2%。根据30个工作日的测定，批间变异系数为6.3%。将本方法与一种特定的气相色谱-质谱分析方法进行了比较，结果表明两种方法具有很好的一致性。一次层析运行需要不到16分钟。用于血浆和10分钟。为了一个标准。
A rapid, yet highly reliable, procedure for determination of homovanillic acid (HVA) in plasma is described. After precipitation of proteins with perchloric acid, separation of sample components is directly achieved with high performance liquid chromatography on a reversed-phase column (C8), followed by quantitation based on electrochemical detection. The sensitivity of this method is 0.5 pmol/injection. Detector response is linear from the limit of detection to at least 0.5 nmol/injection. The intra-assay coefficient of variation is 2.2% in the concentration range of 50-150 pmol/ml plasma. The inter-assay coefficient of variation is 6.3%, based on determinations on 30 working days. A comparison of the present method and a specific gas chromatographic-mass spectrometric assay showed good agreement between the two procedures. One chromatographic run requires less than 16 min. for plasma and 10 min. for a standard.
人血浆中的儿茶酚胺代谢物作为脑功能的指标：异喹啉的影响。
影响因子：
6.1
作者：
Swann,AC;Maas,JW;Hattox,SE;Landis,H
通讯作者：
Landis,H