Mesenchymal stromal/stem cell (MSC)-derived exosomes in clinical trials.
Mesenchymal stromal/stem cell (MSC)-derived exosomes in clinical trials.
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DOI：
10.1186/s13287-023-03287-7
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发表时间：
2023-04-07
影响因子：
7.5
通讯作者：
中科院分区：
文献类型：
--
作者：
间充质基质/干细胞（MSC）由于其强大的免疫调节和再生特性而广泛用于细胞治疗。它们的旁分泌活性是有助于其功效的最重要特征之一。最近，已经证明，通过细胞外囊泡，特别是外来体产生的各种因子，在实验模型中输注后控制MSC的主要功效。与MSC本身相比，MSC衍生的外泌体（MSC-Exos）通过有效地减少不利的副作用（例如输注相关的毒性）提供了显著的优势。MSC-Exos正在成为一种有前途的无细胞治疗工具，越来越多的临床研究开始评估MSC-Exos在不同疾病中的治疗效果。在这篇综述中，我们总结了正在进行和已完成的使用MSC-Exos进行免疫调节、再生医学、基因递送等的临床研究。此外，我们总结了这些研究中使用的MSC-Exos生产方法，重点是MSC来源，MSC-Exos分离方法，表征，剂量和给药途径。最后，我们讨论了目前的挑战和未来的方向外泌体利用在不同的临床研究作为一种新的治疗策略。
Mesenchymal stromal/stem cells (MSCs) are widely utilized in cell therapy because of their robust immunomodulatory and regenerative properties. Their paracrine activity is one of the most important features that contribute to their efficacy. Recently, it has been demonstrated that the production of various factors via extracellular vesicles, especially exosomes, governs the principal efficacy of MSCs after infusion in experimental models. Compared to MSCs themselves, MSC-derived exosomes (MSC-Exos) have provided significant advantages by efficiently decreasing unfavorable adverse effects, such as infusion-related toxicities. MSC-Exos is becoming a promising cell-free therapeutic tool and an increasing number of clinical studies started to assess the therapeutic effect of MSC-Exos in different diseases. In this review, we summarized the ongoing and completed clinical studies using MSC-Exos for immunomodulation, regenerative medicine, gene delivery, and beyond. Additionally, we summarized MSC-Exos production methods utilized in these studies with an emphasis on MSCs source, MSC-Exos isolation methods, characterization, dosage, and route of administration. Lastly, we discussed the current challenges and future directions of exosome utilization in different clinical studies as a novel therapeutic strategy.
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DOI：
10.1007/s00103-021-03373-2
发表时间：
2021-08
期刊：
Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
影响因子：
--
作者：
Dannewitz B;Holtfreter B;Eickholz P
通讯作者：
Eickholz P
影响因子：
3.3
作者：
Cho, Byong Seung;Lee, Jinah;Yi, Yong Weon
通讯作者：
Yi, Yong Weon
影响因子：
6
作者：
Doeppner, Thorsten R.;Herz, Josephine;Hermann, Dirk M.
通讯作者：
Hermann, Dirk M.
影响因子：
--
作者：
De-Paula, Vanessa J;Radanovic, Marcia;Forlenza, Orestes V
通讯作者：
Forlenza, Orestes V
影响因子：
6.5
作者：
Dynoodt, Peter;Mestdagh, Pieter;Van Gele, Mireille J. L.
通讯作者：
Van Gele, Mireille J. L.