Fibroblast-Conditioned Media Enhance the Yield of Microglia Isolated from Mixed Glial Cultures.
Fibroblast-Conditioned Media Enhance the Yield of Microglia Isolated from Mixed Glial Cultures.
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DOI：
10.1007/s10571-022-01193-9
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发表时间：
2023-01
影响因子：
4
通讯作者：
中科院分区：
文献类型：
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小胶质细胞是中枢神经系统（CNS）的主要免疫细胞，并且包括用于研究CNS病症中的炎症机制的各种模型系统。目前，振荡和温和的胰蛋白酶消化是广泛使用的小胶质细胞培养方法;然而，培养小胶质细胞的问题包括产量低和耗时的过程。在本研究中，我们用含25%成纤维细胞条件培养基（F-CM）的培养基代替正常培养基（NM）培养混合胶质细胞，并比较这两种方法获得的小胶质细胞。我们发现F-CM显著提高了小胶质细胞的产量和纯度，并减少了混合胶质细胞的总培养时间。从F-CM组获得的小胶质细胞表现出较长的分枝形态比从NM组，但没有观察到的细胞大小的差异。两组的小胶质细胞具有相似的吞噬功能和基线表型标志物。这两种方法都产生了小胶质细胞对各种刺激如脂多糖（LPS）、干扰素-γ（IFN-γ）和白细胞介素-4（IL-4）的反应。目前的结果表明，F-CM影响混合胶质细胞培养中的原代小胶质细胞的生长。这种方法可以在短时间内产生高产量的原代小胶质细胞，可能是一种方便的方法，为研究人员研究炎症机制和一些CNS疾病。
Microglia are the main immune cells of the central nervous system (CNS) and comprise various model systems used to investigate inflammatory mechanisms in CNS disorders. Currently, shaking and mild trypsinization are widely used microglial culture methods; however, the problems with culturing microglia include low yield and a time-consuming process. In this study, we replaced normal culture media (NM) with media containing 25% fibroblast-conditioned media (F-CM) to culture mixed glia and compared microglia obtained by these two methods. We found that F-CM significantly improved the yield and purity of microglia and reduced the total culture time of mixed glia. The microglia obtained from the F-CM group showed longer ramified morphology than those from the NM group, but no difference was observed in cell size. Microglia from the two groups had similar phagocytic function and baseline phenotype markers. Both methods yielded microglia were responsive to various stimuli such as lipopolysaccharide (LPS), interferon-γ (IFN-γ), and interleukin-4 (IL-4). The current results suggest that F-CM affect the growth of primary microglia in mixed glia culture. This method can produce a high yield of primary microglia within a short time and may be a convenient method for researchers to investigate inflammatory mechanisms and some CNS disorders.
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影响因子：
5.1
作者：
Fu, Ruying;Shen, Qingyu;Xu, Pengfei;Luo, Jin Jun;Tang, Yamei
通讯作者：
Tang, Yamei
影响因子：
9.3
作者：
Cherry JD;Olschowka JA;O'Banion MK
通讯作者：
O'Banion MK
影响因子：
3.3
作者：
Costa, Roberta;Bergwerf, Irene;Ponsaerts, Peter
通讯作者：
Ponsaerts, Peter
DOI：
10.1016/0090-1229(91)90038-c
发表时间：
1991-06-01
期刊：
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY
影响因子：
--
作者：
HASSAN, NF;PRAKASH, K;DOUGLAS, SD
通讯作者：
DOUGLAS, SD
影响因子：
4.2
作者：
Ju, Lili;Zeng, Hui;Xu, Qunyuan
通讯作者：
Xu, Qunyuan