In Vivo Murine Model of Leukemia Cell-Induced Spinal Bone Destruction.
In Vivo Murine Model of Leukemia Cell-Induced Spinal Bone Destruction.
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白血病细胞诱导的脊柱骨破坏的体内小鼠模型
DOI：
10.1155/2017/3521481
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发表时间：
2017
影响因子：
--
通讯作者：
Chang G
中科院分区：
文献类型：
--
作者：
Chen JJ;Zhou W;Cai N;Chang G
溶骨性骨损害可能是白血病骨渗透或白血病细胞释放的炎症因子引起的局部骨破坏的结果。破坏性的骨骼损伤会对白血病患者的生活质量产生负面影响，导致无法忍受的疼痛，在某些情况下还会导致肢体瘫痪。然而，白血病细胞产生破坏性骨损伤的机制以及治疗方法对溶骨性损伤的影响尚未完全阐明，因此，有望从体内模型中受益。为此，通过逆转录病毒介导的成分活性(CA)STAT5表达转化HL-60细胞，并通过尾静脉注射到非肥胖糖尿病(NOD)/SCID小鼠。三周后，对腰椎进行组织细胞计量学分析。异种移植小鼠在2-3周内出现后肢瘫痪，这与白血病细胞髓外侵袭造成的脊髓骨质破坏的后果一致。体内模型将提高对髓系白血病细胞引起的溶骨性骨损害的认识和治疗。
Osteolytic bone lesions can be a consequence of leukemic bone infiltration or focal bone destruction by inflammatory factors released from leukemic cells. Destructive bone lesions have a negative impact on the quality of life of leukemia patients, causing unbearable pain and, in some cases, limb paralysis. However, the mechanism, by which leukemic cells produce destructive bone lesions, and the effect of therapeutics on osteolytic lesions have not been fully elucidated yet and, thus, stand to benefit from an in vivo model. To that end, HL-60 cells were transformed by retrovirus-mediated constitutively active (CA) STAT5 expression and injected into nonobese diabetic (NOD)/SCID mice via the tail vein. After three weeks, lumbar spines were subjected to histocytometric analysis. Xenograft mice developed hind limb paralysis in 2-3 weeks, which was consistent with the consequences of spinal bone destruction by extramedullary invasion of leukemia cells. The in vivo model will improve the understanding and treatment of osteolytic bone lesions caused by myeloid leukemic cells.
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DOI：
10.7759/cureus.639
发表时间：
2016-06-13
期刊：
Cureus
影响因子：
--
作者：
Chambers I;Truong P;Kallail KJ;Palko W
通讯作者：
Palko W
影响因子：
20.3
作者：
Lemoli, RM;Fortuna, A;Tura, S
通讯作者：
Tura, S
影响因子：
20.3
作者：
Ye, Dan;Wolff, Nicholas;Ilaria, Robert L., Jr.
通讯作者：
Ilaria, Robert L., Jr.
影响因子：
2.8
作者：
Jurisic, Vladimir;Terzic, Tatjana;Colovic, Milica
通讯作者：
Colovic, Milica
影响因子：
20.3
作者：
Harir, Noria;Pecquet, Christian;Gouilleux, Fabrice
通讯作者：
Gouilleux, Fabrice