Knockdown of KLF9 promotes the differentiation of both intramuscular and subcutaneous preadipocytes in goat
Knockdown of KLF9 promotes the differentiation of both intramuscular and subcutaneous preadipocytes in goat
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KLF9的敲低促进山羊肌内和皮下前脂肪细胞的分化
DOI：
10.1080/09168451.2020.1767497
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发表时间：
2020-05
期刊：
影响因子：
--
通讯作者：
Jiangjiang Zhu
中科院分区：
文献类型：
--
作者：
Qing Xu;Yaqiu Lin;Yong Wang;Wenlin Bai;Jiangjiang Zhu
摘要据报道，KLF 9可促进3 T3-L1细胞和猪的脂肪细胞分化。然而，KLF 9在山羊脂肪细胞分化中的作用尚不清楚。本研究中，KLF 9在山羊皮下和肌内前脂肪细胞分化过程中的表达谱存在差异。沉默KLF 9基因后，两种脂肪细胞中的脂滴均增加。在皮下前脂肪细胞中，沉默KLF 9后，C/EBPβ、PPARγ、LPL、KLF 1 -2、KLF 5和KLF 17基因表达上调，KLF 12、KLF 4和KLF 13基因表达下调。肌内前脂肪细胞中aP 2、C/EBPα、KLF 2 -3、KLF 5、KLF 7基因表达上调，Pref-1基因表达下调。此外，KLF 9的结合位点存在于aP 2、C/EBPα、C/EBPβ、LPL和Pref-1的启动子中。KLF 9对山羊肌内和皮下前脂肪细胞的分化均起负性作用，但作用机制可能不同。图摘要敲低KLF 9可促进肌内和皮下前脂肪细胞的分化，但作用机制可能不同。
ABSTRACT KLF9 is reported to promote adipocyte differentiation in 3T3-L1 cells and pigs. However, the roles of KLF9 in adipocytes differentiation of goat remain unknown. In this study, the expression profiles of KLF9 were different between subcutaneous and intramuscular preadipocytes of goat during differentiation process. After silencing KLF9 gene, the lipid droplets were increased in both two types of adipocytes. In subcutaneous preadipocyte with silencing KLF9, the expressions of C/EBPβ, PPARγ, LPL, KLF1-2, KLF5, and KLF17 genes were up-regulated, while KLF12, KLF4, and KLF13 genes were down-regulated in expression level. In intramuscular preadipocyte, aP2, C/EBPα, KLF2-3, KLF5, and KLF7 gene were up-regulated, and Pref-1 gene was down-regulated. In addition, the binding sites of KLF9 existed in the promoters of aP2, C/EBPα, C/EBPβ, LPL and Pref-1. Taken together, KLF9 play a negative role in the differentiation of both intramuscular and subcutaneous preadipocytes in goats, but the functional mechanism may be different. GRAPHICAL ABSTRACT Knockdown KLF9 promotes differentiation of intramuscular and subcutaneous preadipocyte, however, the mechanism of action might be different.
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DOI：
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