Transcriptional and functional profiling defines human small intestinal macrophage subsets.
Transcriptional and functional profiling defines human small intestinal macrophage subsets.
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DOI：
10.1084/jem.20170057
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发表时间：
2018-02-05
期刊：
影响因子：
--
通讯作者：
Jahnsen FL
中科院分区：
文献类型：
--
作者：
Bujko A;Atlasy N;Landsverk OJB;Richter L;Yaqub S;Horneland R;Øyen O;Aandahl EM;Aabakken L;Stunnenberg HG;Bækkevold ES;Jahnsen FL
Bujko等人描述了人小肠中巨噬细胞的四个不同亚群，它们在移植的十二指肠中被完全取代。这些亚群在其表型、功能和转录组谱中显示出渐进的变化，表明它们代表了组织中单核细胞衍生的巨噬细胞成熟的阶段。巨噬细胞（Mfs）在维持肠道免疫稳态中起重要作用，但对人类肠道Mfs的起源和异质性的研究很少。在这里，我们确定了四个不同的MF亚群在人类小肠（SI）。在十二指肠移植物的营业额的评估显示，所有的Mf亚群完全取代随着时间的推移，Mf 1和Mf 2，表型相似的外周血单核细胞（PBMos），主要是在3周内更换，而两个子集的功能成熟的Mfs，Mf 3和Mf 4，表现出显着较慢的更换。Mf 3和Mf 4在SI中的定位不同; Mf 3在粘膜固有层中形成致密的网络，而Mf 4在粘膜下层中富集。转录分析表明，所有的Mf亚群显着不同的PBMos和树突状细胞。与PBMos相比，Mf亚群对促炎刺激的反应性降低，但精通颗粒和可溶性物质的内吞作用。这些数据提供了对人SIMf群体的综合分析，并表明与PBM 0的后代-后代关系。
Bujko et al. describe four distinct subsets of macrophages in human small intestine that are completely replaced in transplanted duodenum. These subsets show graduated changes in their phenotypes, function, and transcriptome profiles, suggesting that they represent stages of monocyte-derived macrophage maturation in tissue. Macrophages (Mfs) are instrumental in maintaining immune homeostasis in the intestine, yet studies on the origin and heterogeneity of human intestinal Mfs are scarce. Here, we identified four distinct Mf subpopulations in human small intestine (SI). Assessment of their turnover in duodenal transplants revealed that all Mf subsets were completely replaced over time; Mf1 and Mf2, phenotypically similar to peripheral blood monocytes (PBMos), were largely replaced within 3 wk, whereas two subsets with features of mature Mfs, Mf3 and Mf4, exhibited significantly slower replacement. Mf3 and Mf4 localized differently in SI; Mf3 formed a dense network in mucosal lamina propria, whereas Mf4 was enriched in submucosa. Transcriptional analysis showed that all Mf subsets were markedly distinct from PBMos and dendritic cells. Compared with PBMos, Mf subpopulations showed reduced responsiveness to proinflammatory stimuli but were proficient at endocytosis of particulate and soluble material. These data provide a comprehensive analysis of human SI Mf population and suggest a precursor-progeny relationship with PBMos.
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影响因子：
3.7
作者：
Beitnes, A. -C. R.;Raki, M.;Jahnsen, F. L.
通讯作者：
Jahnsen, F. L.
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1084/jem.20081633
发表时间：
2009-02-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Haniffa M;Ginhoux F;Wang XN;Bigley V;Abel M;Dimmick I;Bullock S;Grisotto M;Booth T;Taub P;Hilkens C;Merad M;Collin M
通讯作者：
Collin M
影响因子：
20.3
作者：
Kim, Ki-Wook;Vallon-Eberhard, Alexandra;Jung, Steffen
通讯作者：
Jung, Steffen
IL-10产生的肠道巨噬细胞通过限制IL-23合成来防止过度的抗菌先天免疫。
影响因子：
16.6
作者：
Krause, Petra;Morris, Venetia;Greenbaum, Jason A.;Park, Yoon;Bjoerheden, Unni;Mikulski, Zbigniew;Muffley, Tracy;Shui, Jr-Wen;Kim, Gisen;Cheroutre, Hilde;Liu, Yun-Cai;Peters, Bjoern;Kronenberg, Mitchell;Murai, Masako
通讯作者：
Murai, Masako