Using the Localized Surface Plasmon Resonance of Gold Nanoparticles to Monitor Lipid Membrane Assembly and Protein Binding.
Using the Localized Surface Plasmon Resonance of Gold Nanoparticles to Monitor Lipid Membrane Assembly and Protein Binding.
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DOI：
10.1021/jp406013q
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发表时间：
2013-12-19
期刊：
影响因子：
--
通讯作者：
Reed SM
中科院分区：
文献类型：
--
作者：
Messersmith RE;Nusz GJ;Reed SM
金纳米颗粒为制备具有明确曲率的支持脂质层提供了模板。在这里，我们利用金纳米粒子的局部表面等离子体共振（LSPR）作为传感器来监测纳米粒子上脂质层的制备。LSPR对纳米颗粒表面的直接环境非常敏感，它被用来监测脂质涂层和随后的支持双分子层向杂化膜的转化，杂化膜的外层是脂质叶，内部是含有疏水烷硫醇的叶。我们证明了十硫醇和丙硫醇都能够形成杂化膜，并且在较短的硫醇上形成的膜可以使用β-巯基乙醇从金和脂质叶上剥离。当使用较短的硫醇（37.8±1.5 nm / RI单位）时，纳米粒子LSPR对周围折射率（RI）的敏感性大于使用较长的硫醇（27.5±0.5 nm / RI单位）时。最后，c反应蛋白与膜的结合使用该传感器进行测量，允许观察蛋白质-膜和纳米颗粒-纳米颗粒的相互作用，而不需要对蛋白质或脂质进行化学标记。
Gold nanoparticles provide a template for preparing supported lipid layers with well-defined curvature. Here, we utilize the localized surface plasmon resonance (LSPR) of gold nanoparticles as a sensor for monitoring the preparation of lipid layers on nanoparticles. The LSPR is very sensitive to the immediate surroundings of the nanoparticle surface and it is used to monitor the coating of lipids and subsequent conversion of a supported bilayer to a hybrid membrane with an outer lipid leaflet and an inner leaflet containing hydrophobic alkanethiol. We demonstrate that both decanethiol and propanethiol are able to form hybrid membranes and that the membrane created over the shorter thiol can be stripped from the gold along with the lipid leaflet using β-mercaptoethanol. The sensitivity of the nanoparticle LSPR to the refractive index (RI) of its surroundings is greater when the shorter thiol is used (37.8 ± 1.5 nm per RI unit) than when the longer thiol is used (27.5 ± 0.5 nm per RI unit). Finally, C-reactive protein binding to the membrane is measured using this sensor allowing observation of both protein-membrane and nanoparticle-nanoparticle interactions without chemical labeling of protein or lipids.
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