RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation.
RNA helicase-mediated regulation of snoRNP dynamics on pre-ribosomes and rRNA 2'-O-methylation.
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DOI：
10.1093/nar/gkab159
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发表时间：
2021-04-19
影响因子：
14.9
通讯作者：
Bohnsack MT
中科院分区：
文献类型：
--
作者：
Aquino GRR;Krogh N;Hackert P;Martin R;Gallesio JD;van Nues RW;Schneider C;Watkins NJ;Nielsen H;Bohnsack KE;Bohnsack MT
RNA解旋酶通过其在重塑核糖核蛋白复合物（RNP）如前核糖体中的功能在RNA代谢的各个方面发挥重要作用。在这里，我们表明，DEAD盒解旋酶Dbp 3是有效加工U18和U24内含子编码的snoRNA和25 S核糖体RNA（rRNA）序列内多个位点的2′-O-甲基化所必需的。此外，在Dbp 3不存在的情况下，许多盒C/D snoRNP在前核糖体上积累。许多引导Dbp 3依赖性rRNA修饰的snoRNA具有重叠的前rRNA碱基配对位点，因此与前核糖体形成相互排斥的相互作用。分析这些snoRNA在前核糖体相关和“游离”池之间的分布表明，许多snoRNA几乎完全与前核糖体复合物相关。我们的数据表明，当Dbp 3缺乏时，这种snoRNP在前核糖体上的保留可能通过降低snoRNP的回收效率和抑制snoRNP接近近端靶位点来阻碍rRNA 2′-O-甲基化。在相邻位点处的亚化学计量rRNA修饰的观察表明，引导这种修饰的snoRNP可能与其前rRNA靶位点随机相互作用，而不是分层相互作用。总之，我们的数据提供了新的见解snoRNP对前核糖体复合物的动力学和重塑事件发生在核糖体组装的早期阶段。
RNA helicases play important roles in diverse aspects of RNA metabolism through their functions in remodelling ribonucleoprotein complexes (RNPs), such as pre-ribosomes. Here, we show that the DEAD box helicase Dbp3 is required for efficient processing of the U18 and U24 intron-encoded snoRNAs and 2′-O-methylation of various sites within the 25S ribosomal RNA (rRNA) sequence. Furthermore, numerous box C/D snoRNPs accumulate on pre-ribosomes in the absence of Dbp3. Many snoRNAs guiding Dbp3-dependent rRNA modifications have overlapping pre-rRNA basepairing sites and therefore form mutually exclusive interactions with pre-ribosomes. Analysis of the distribution of these snoRNAs between pre-ribosome-associated and ‘free’ pools demonstrated that many are almost exclusively associated with pre-ribosomal complexes. Our data suggest that retention of such snoRNPs on pre-ribosomes when Dbp3 is lacking may impede rRNA 2′-O-methylation by reducing the recycling efficiency of snoRNPs and by inhibiting snoRNP access to proximal target sites. The observation of substoichiometric rRNA modification at adjacent sites suggests that the snoRNPs guiding such modifications likely interact stochastically rather than hierarchically with their pre-rRNA target sites. Together, our data provide new insights into the dynamics of snoRNPs on pre-ribosomal complexes and the remodelling events occurring during the early stages of ribosome assembly.
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影响因子：
7.3
作者：
Henras, Anthony K.;Plisson-Chastang, Celia;O'Donohue, Marie-Francoise;Chakraborty, Anirban;Gleizes, Pierre-Emmanuel
通讯作者：
Gleizes, Pierre-Emmanuel
HAS1调节连续的成熟和处理步骤，用于组装60S核糖体亚基。
影响因子：
14.9
作者：
Dembowski JA;Kuo B;Woolford JL Jr
通讯作者：
Woolford JL Jr
影响因子：
16.6
作者：
Birkedal, Ulf;Christensen-Dalsgaard, Mikkel;Nielsen, Henrik
通讯作者：
Nielsen, Henrik
影响因子：
11.4
作者：
Giorgi, C;Fatica, A;Bozzoni, I
通讯作者：
Bozzoni, I
影响因子：
48
作者：
Breslow, David K.;Cameron, Dale M.;Weissman, Jonathan S.
通讯作者：
Weissman, Jonathan S.