An Ointment Consisting of the Phage Lysin LysGH15 and Apigenin for Decolonization of Methicillin-Resistant Staphylococcus aureus from Skin Wounds.
An Ointment Consisting of the Phage Lysin LysGH15 and Apigenin for Decolonization of Methicillin-Resistant Staphylococcus aureus from Skin Wounds.
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一种由噬菌体溶素 LysGH15 和芹菜素组成的软膏，用于去除皮肤伤口中的耐甲氧西林金黄色葡萄球菌
DOI：
10.3390/v10050244
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发表时间：
2018-05-06
期刊：
影响因子：
--
通讯作者：
Gu J
中科院分区：
文献类型：
--
作者：
Cheng M;Zhang L;Zhang H;Li X;Wang Y;Xia F;Wang B;Cai R;Guo Z;Zhang Y;Ji Y;Sun C;Feng X;Lei L;Yang Y;Han W;Gu J
金黄色葡萄球菌(S.aureus)是一种常见且危险的病原体，可引起多种传染病。皮肤损伤，如烧伤创面，有很高的金黄色葡萄球菌定植和感染的风险，这会增加发病率和死亡率。噬菌体裂解酶LysGH15对耐甲氧西林金黄色葡萄球菌(MRSA)和甲氧西林敏感金黄色葡萄球菌(MSSA)具有高效的裂解活性。芹菜素(API)能显著降低金黄色葡萄球菌引起的兔红细胞溶血，具有抗炎作用。将LysGH15和API添加到Aquaphor中，形成LysGH15-API-Aquaphor(LAA)软膏。LAA软膏同时显示出对金黄色葡萄球菌的杀菌活性和抑制溶血作用。在用LAA治疗的MRSA感染皮肤伤口的小鼠模型中，治疗后18小时的平均细菌菌落计数降至约102 cfu/mg(96 h时细菌检测不到)，而未治疗的小鼠的平均细菌计数约为105 cfu/mg组织。在小鼠模型中，LAA软膏还降低了促炎细胞因子(肿瘤坏死因子-α、IL-1β和干扰素-γ)的水平，并加速了伤口的愈合。这些数据证明了LysGH15和API的组合用作局部抗菌剂对抗金黄色葡萄球菌的潜在功效。
Staphylococcus aureus (S. aureus) is a common and dangerous pathogen that causes various infectious diseases. Skin damage, such as burn wounds, are at high risk of Staphylococcus aureus colonization and infection, which increases morbidity and mortality. The phage lysin LysGH15 exhibits highly efficient lytic activity against methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains. Apigenin (api) significantly decreases haemolysis of rabbit erythrocytes caused by S. aureus and shows anti-inflammatory function. LysGH15 and api were added to Aquaphor to form an LysGH15-api-Aquaphor (LAA) ointment. The LAA ointment simultaneously exhibited bactericidal activity against S. aureus and inhibited haemolysis. In an LAA-treated mouse model of an MRSA-infected skin wound, the mean bacterial colony count decreased to approximately 102 CFU/mg at 18 h after treatment (and the bacteria became undetectable at 96 h), whereas the mean count in untreated mice was approximately 105 CFU/mg of tissue. The LAA ointment also reduced the levels of pro-inflammatory cytokines (TNF-α, IL-1β, and IFN-γ) and accelerated wound healing in the mouse model. These data demonstrate the potential efficacy of a combination of LysGH15 and api for use as a topical antimicrobial agent against S. aureus.
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