Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus.
Identification and characterization of vp7 gene in Bombyx mori cytoplasmic polyhedrosis virus.
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家蚕胞质多角体病毒vp7基因的鉴定和表征。
DOI：
10.1016/j.gene.2017.06.048
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发表时间：
2017-09-05
期刊：
影响因子：
3.5
通讯作者：
Gong C
中科院分区：
文献类型：
--
作者：
He L;Hu X;Zhu M;Liang Z;Chen F;Zhu L;Kuang S;Cao G;Xue R;Gong C
家蚕质型多角体病毒（BmCPV）基因组由10个双链RNA片段（S1-S10）组成。片段7（S7）编码被认为是结构蛋白的50 kDa蛋白。p50在病毒生活史中的表达模式和功能尚不清楚。本研究以家蚕质型多角体病毒为研究对象，通过免疫小鼠制备病毒结构蛋白7（VP 7）多克隆抗体，探讨家蚕质型多角体病毒中是否存在VP 7基因编码的小分子蛋白。通过在BmN细胞中过表达vp 7基因来研究其表达模式。除VP 7外，用蛋白质印迹技术鉴定了补充条带。免疫印迹法检测病毒粒子、BmCPV感染细胞和中肠。在相应样品中分别检测到4、2和5条条带。利用RNA干扰技术降低家蚕vp 7基因的表达水平，可抑制家蚕中肠和培养细胞中BmCPV基因组的复制。在免疫沉淀实验中，用VP 7多克隆抗血清在BmCPV感染的中肠组织中检测到一条较短的蛋白带，并对该蛋白带进行质谱分析，结果表明在家蚕中鉴定出一个候选的相互作用蛋白（VP 7 voltage-dependent anion-selective channel-like isoform，VDAC）。我们的结论是，新的病毒产物产生的泄漏扫描机制和VDAC可能是一个相互作用的蛋白与VP 7。BmCPV中存在小分子VP 7蛋白。通过降低vp 7基因的表达，降低BmCPV基因组的复制。这种新的产品是用泄漏扫描机制产生的。
The genome of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) contains 10 double stranded RNA segments (S1–S10). The segment 7 (S7) encodes 50 kDa protein which is considered as a structural protein. The expression pattern and function of p50 in the virus life cycle are still unclear. In this study, the viral structural protein 7 (VP7) polyclonal antibody was prepared with immunized mouse to explore the presence of small VP7 gene-encoded proteins in Bombyx mori cytoplasmic polyhedrosis virus. The expression pattern of vp7 gene was investigated by its overexpression in BmN cells. In addition to VP7, supplementary band was identified with western blotting technique. The virion, BmCPV infected cells and midguts were also examined using western blotting technique. 4, 2 and 5 bands were detected in the corresponding samples, respectively. The replication of BmCPV genome in the cultured cells and midgut of silkworm was decreased by reducing the expression level of vp7 gene using RNA interference. In immunoprecipitation experiments, using a polyclonal antiserum directed against the VP7, one additional shorter band in BmCPV infected midguts was detected, and then the band was analyzed with mass spectrum (MS), the MS results showed thatone candidate interacted protein (VP7 voltage-dependent anion-selective channel-like isoform, VDAC) was identified from silkworm. We concluded that the novel viral product was generated with a leaky scanning mechanism and the VDAC may be an interacted protein with VP7. The small VP7 protein was present in BmCPV. The replication of BmCPV genome was decreased by reducing vp7 gene expression. This novel product was generated with a leaky scanning mechanism.
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影响因子：
13.8
作者：
Hiller, Sebastian;Abramson, Jeff;Mannella, Carmen;Wagner, Gerhard;Zeth, Kornelius
通讯作者：
Zeth, Kornelius
DOI：
10.1073/pnas.75.3.1086
发表时间：
1978-01-01
影响因子：
11.1
作者：
FURUICHI, Y
通讯作者：
FURUICHI, Y
影响因子：
3.7
作者：
Liprandi, F;Moros, Z;Cohen, J
通讯作者：
Cohen, J
影响因子：
3.8
作者：
Qanungo, KR;Kundu, SC;Ghosh, AK
通讯作者：
Ghosh, AK
影响因子：
11.4
作者：
CURRAN, J;KOLAKOFSKY, D
通讯作者：
KOLAKOFSKY, D