Determination and metabolism of dithiol-chelating agents: electrolytic and chemical reduction of oxidized dithiols in urine.
Determination and metabolism of dithiol-chelating agents: electrolytic and chemical reduction of oxidized dithiols in urine.
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二硫醇螯合剂的测定和代谢：尿液中氧化二硫醇的电解和化学还原。
DOI：
10.1016/0003-2697(87)90633-6
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发表时间：
1987
影响因子：
2.9
通讯作者：
Aposhian,HV
中科院分区：
文献类型：
--
作者：
Maiorino,RM;Barry,TJ;Aposhian,HV
用化学还原法和电解还原法测定了人尿中二硫醇螯合剂meso-2，3-二巯基丁二酸（DMSA）和2，3-二巯基丙烷-1-磺酸（DMPS）的氧化态。给予DMSA或DMPS的人的尿液用电解、二硫苏糖醇或四氢硼酸钠（NaBH 4）处理。用monobromobimane对SH基团进行衍生化，以测定未改变的二硫醇。通过还原，然后用monobromobimane衍生化，测定总二硫醇（未改变和氧化）。通过HPLC和荧光法鉴定和定量双满烷衍生物。虽然所有三种还原方法得到了相似的结果，但基于日常重现性和标准品回收率，建议电解还原氧化DMSA和用NaBH 4化学还原氧化DMPS。减少后，4倍增加DMSA和20倍增加DMPS被发现在尿液中的DMSA或DMPS口服剂量后12小时。这些新的二硫醇及其氧化形式的测定方法，应导致更好地了解这些日益重要的口服有效的螯合剂的代谢特性。
The presence of oxidized species of the dithiol-chelating agents, meso-2,3-dimercaptosuccinic acid (DMSA) and 2,3-dimercaptopropane-1-sulfonic acid (DMPS), in human urine was determined by chemical and electrolytic reduction methods. Urine from a human given either DMSA or DMPS was treated with electrolysis, dithiothreitol, or sodium tetrahydridoborate (NaBH4). The SH groups were derivatized with monobromobimane for the determination of unaltered dithiols. Total dithiol (unaltered and oxidized) was determined by reduction followed by derivatization with monobromobimane. The bimane derivatives were identified and quantified by HPLC and fluorescence. Although all three reduction methods gave similar results, electrolytic reduction of oxidized DMSA and chemical reduction with NaBH4of oxidized DMPS are recommended based upon both day to day reproducibility and recovery of standards. After reduction a 4-fold increase in DMSA and a 20-fold increase in DMPS were found in urine by 12 h after an oral dose of DMSA or DMPS. These new methods for the determination of dithiols and their oxidized forms should lead to a better understanding of the metabolic properties of these increasingly important orally effective chelating agents.
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影响因子：
4.8
作者：
V. G. Janolino;H. Swaisgood
通讯作者：
H. Swaisgood
螯合剂 2,3-二巯基丙烷-1-磺酸盐与体外红细胞的相互作用。
影响因子：
5.1
作者：
D. Wildenauer;H. Reuther;N. Weger
通讯作者：
N. Weger
二硫醇金属结合剂影响兔子体内亚砷酸盐的生物转化。
影响因子：
3.8
作者：
Maiorino,RM;Aposhian,HV
通讯作者：
Aposhian,HV
DOI：
10.1016/s0006-291x(75)80329-9
发表时间：
1975-01-01
影响因子：
3.1
作者：
CHANG, TSK;MORTON, B
通讯作者：
MORTON, B
β-巯基丙酮酸转硫酶铜含量的研究
影响因子：
4.8
作者：
C. Hamer;A. Morell;I. Scheinberg
通讯作者：
I. Scheinberg