Super-resolved 3D tracking of cargo transport through nuclear pore complexes.
Super-resolved 3D tracking of cargo transport through nuclear pore complexes.
复制标题
DOI：
10.1038/s41556-021-00815-6
复制
发表时间：
2022-01
影响因子：
21.3
通讯作者：
Musser SM
中科院分区：
文献类型：
--
作者：
Chowdhury R;Sau A;Musser SM
核孔复合体(NPC)嵌入核膜(NE)，介导细胞质和核质之间的快速、选择性和双向运输。破译这一过程的机制和动力学面临着对高空间和时间精度的需求的挑战。我们在此报告了一种多色成像方法，该方法能够相对于NPC支架的超分辨八角形双环结构直接三维可视化货物运输轨迹。实验和模拟相结合的分析证实，这种方法的成功是因为渗透细胞内的NPC具有很高的位置稳定性。代表不同核运输受体(NTR)途径的两个货物复合体的沙漏状转运管道表明，快速通过NPC支架上或附近的渗透屏障进行迁移。货物复合体的结合部位从孔口延伸超过100 nm，与结合NTRs的FG多肽的广泛分布一致。
Nuclear pore complexes (NPCs) embedded within the nuclear envelope (NE) mediate rapid, selective, and bidirectional traffic between the cytoplasm and the nucleoplasm. Deciphering the mechanism and dynamics of this process is challenged by the need for high spatial and temporal precision. We report here a multi-color imaging approach that enables direct 3D visualization of cargo transport trajectories relative to a super-resolved octagonal double-ring structure of the NPC scaffold. The success of this approach is enabled by the high positional stability of NPCs within permeabilized cells, as verified by a combined experimental and simulation analysis. Hourglass-shaped translocation conduits for two cargo complexes representing different nuclear transport receptor (NTR) pathways indicates rapid migration through the permeability barrier on or near the NPC scaffold. Binding sites for cargo complexes extend over 100 nm from the pore openings, consistent with a wide distribution of the FG-polypeptides that bind NTRs.
登录
查看更多内容
DOI：
10.1073/pnas.1702226114
发表时间：
2017-09-26
影响因子：
11.1
作者：
Chu, Fang-Yi;Haley, Shannon C.;Zidovska, Alexandra
通讯作者：
Zidovska, Alexandra
影响因子：
64.8
作者：
Kim SJ;Fernandez-Martinez J;Nudelman I;Shi Y;Zhang W;Raveh B;Herricks T;Slaughter BD;Hogan JA;Upla P;Chemmama IE;Pellarin R;Echeverria I;Shivaraju M;Chaudhury AS;Wang J;Williams R;Unruh JR;Greenberg CH;Jacobs EY;Yu Z;de la Cruz MJ;Mironska R;Stokes DL;Aitchison JD;Jarrold MF;Gerton JL;Ludtke SJ;Akey CW;Chait BT;Sali A;Rout MP
通讯作者：
Rout MP
影响因子：
16.6
作者：
Eibauer, Matthias;Pellanda, Mauro;Turgay, Yagmur;Dubrovsky, Anna;Wild, Annik;Medalia, Ohad
通讯作者：
Medalia, Ohad
影响因子：
8
作者：
Fischer, H;Polikarpov, I;Craievich, AF
通讯作者：
Craievich, AF
DOI：
10.1038/7625
发表时间：
1999-04-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Kobe, B
通讯作者：
Kobe, B