Electron transport controls transcription of the glutamine synthetase gene (glnA) from the cyanobacterium Synechocystis sp. PCC 6803
Electron transport controls transcription of the glutamine synthetase gene (glnA) from the cyanobacterium Synechocystis sp. PCC 6803
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电子传递控制来自蓝藻集胞藻属的谷氨酰胺合成酶基因 (glnA) 的转录。
DOI：
10.1007/bf00020231
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发表时间：
1995
影响因子：
5.1
通讯作者：
F. Florencio
中科院分区：
文献类型：
--
作者：
J. Reyes;F. Florencio
集胞藻PCC 6803的glnA基因编码I型谷氨酰胺合成酶（GS），与其他蓝藻glnA基因序列相似性较高。转移到黑暗中后，或与电子传递抑制剂DCMU或DBMIB孵育后，观察到glnA mRNA（约1.6 kb的单一转录物）的量急剧下降。glnA转录水平在再照射5分钟后完全恢复。发现glnA mRNA在光照和黑暗中同样稳定（半衰期约2.5分钟）。与glnA信使不同，GS蛋白的量在黑暗中没有减少。在黑暗中合成glnA转录物需要葡萄糖的存在。此外，glnA转录在集胞藻psbE-psbF突变体缺乏光系统II需要的存在下，即使在光照下生长的葡萄糖。这些观察结果表明，glnA转录的细胞的氧化还原状态的控制下。最后，氮饥饿引起延迟glnA转录在黑暗中的减少，这表明氮和氧化还原控制glnA转录水平之间的连接。
The glnA gene, encoding type I glutamine synthetase (GS) in Synechocystis sp. PCC 6803, showed a high sequence similarity with other cyanobacterial glnA genes. A dramatic decrease in the amount of glnA mRNA, a single transcript of about 1.6 kb, was observed after transfer to darkness, or after incubation with the electron transport inhibitors DCMU or DBMIB. The levels of glnA transcript were fully recovered after 5 min of reillumination. The glnA mRNA was found to be equally stable both in the light and the dark (half-life about 2.5 min). Unlike the glnA messenger, the amount of GS protein was not reduced in the dark. Synthesis of the glnA transcript in the dark required the presence of glucose. In addition, glnA transcription in a Synechocystis psbE-psbF mutant lacking photosystem II required the presence of glucose even when grown in the light. These observations indicate that glnA transcription is under the control of the redox state of the cell. Finally, nitrogen starvation provoked a delay in the decrease of glnA transcript in darkness, suggesting a connection between nitrogen and redox controls of glnA transcript levels.
DOI：
10.1002/j.1460-2075.1987.tb04710.x
发表时间：
1987-01
期刊：
The EMBO Journal
影响因子：
--
作者：
S. Tingey;E. Walker;G. Coruzzi
通讯作者：
S. Tingey;E. Walker;G. Coruzzi
影响因子：
11.6
作者：
EDWARDS, JW;CORUZZI, GM
通讯作者：
CORUZZI, GM