The Application of Microfluidic Technologies in Aptamer Selection.
The Application of Microfluidic Technologies in Aptamer Selection.
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DOI：
10.3389/fcell.2021.730035
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发表时间：
2021
影响因子：
5.5
通讯作者：
Ren K
中科院分区：
文献类型：
--
作者：
Liu Y;Wang N;Chan CW;Lu A;Yu Y;Zhang G;Ren K
适体是单链寡核苷酸（DNA或RNA）的序列，其具有与特定靶分子的潜在结合能力，其越来越多地用作分析、诊断和医学治疗的试剂。适体是通过一种称为指数富集配体系统进化（SELEX）的选择方法产生的。已经开发了许多用于适体选择的SELEX方法。然而，传统的SELEX方法仍然存在劳动强度高、操作效率低、成功率低的问题。因此，具有所需性质的适体的应用受到限制。微流控技术具有成本低、速度快、自动化程度高等优点，已成为快速、高通量筛选核酸适体的有效工具。本文综述了近年来这类微流控系统在核酸适体筛选中的研究进展。比较的选择性能与讨论的原则，结构，操作，以及各种基于微流体的适体选择方法的优点和局限性。
Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligands by exponential enrichment (SELEX). Numerous SELEX methods have been developed for aptamer selections. However, the conventional SELEX methods still suffer from high labor intensity, low operation efficiency, and low success rate. Thus, the applications of aptamer with desired properties are limited. With their advantages of low cost, high speed, and upgraded extent of automation, microfluidic technologies have become promising tools for rapid and high throughput aptamer selection. This paper reviews current progresses of such microfluidic systems for aptamer selection. Comparisons of selection performances with discussions on principles, structure, operations, as well as advantages and limitations of various microfluidic-based aptamer selection methods are provided.
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影响因子：
4.6
作者：
Birch CM;Hou HW;Han J;Niles JC
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