Diversity and Ecological Guild Analysis of the Oil Palm Fungal Microbiome Across Root, Rhizosphere, and Soil Compartments.
Diversity and Ecological Guild Analysis of the Oil Palm Fungal Microbiome Across Root, Rhizosphere, and Soil Compartments.
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DOI：
10.3389/fmicb.2022.792928
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发表时间：
2022
影响因子：
5.2
通讯作者：
Bending GD
中科院分区：
文献类型：
--
作者：
Kirkman ER;Hilton S;Sethuraman G;Elias DMO;Taylor A;Clarkson J;Soh AC;Bass D;Ooi GT;McNamara NP;Bending GD
根际微生物组是植物健康的主要决定因素，它可以直接或间接与宿主相互作用，以促进或抑制生产力。油棕是世界上最重要的农作物之一，占全球植物油产量的三分之一以上。目前，人们对油棕微生物组及其对植物健康和生产力的贡献知之甚少，现有知识几乎完全基于文化依赖性研究。我们调查了马来西亚雪兰莪州 2 年、18 年和 35 年种植园的大块土壤、根际土壤和根部油棕真菌微生物组的多样性和组成。种植园之间的真菌群落表现出显着差异，占群落组成的 19.7%，其中区室（根、根际土壤和土体土壤）和土壤性质（pH、C、N 和 P）分别贡献了群落变化的 6.5% 和 7.2%。与大块土壤相比，根际土壤和根支持不同的群落，根中伞形孢菌、球囊菌和球囊菌显着富集。所有种植园的根部都富含几种假定的植物病原体，包括与 Prospodicola mexicana 和 Pleurostoma sp. 相关的类群。油棕的菌根状态和依赖性尚未确定，使用 18S rRNA 引物，我们发现球囊菌亚门群落组成存在相当大的位点间变异，占变异的 31.2%。有证据表明较老种植园的油棕根部存在肾囊菌亚门群落选择，但隔室对群落组成影响较弱，占变异的 3.9%，而土壤变量占群落变异的 9%。虽然检测到了多种毛霉菌门真菌，但与大块土壤相比，它们在根内表现出非常低的丰度和多样性，并且与与细根内生菌菌根形态相关的类群没有密切关系。许多真菌序列与已建立的属的相似性较低，表明存在大量新颖的多样性，对油棕微生物组内的植物健康具有重要意义。
The rhizosphere microbiome is a major determinant of plant health, which can interact with the host directly and indirectly to promote or suppress productivity. Oil palm is one of the world’s most important crops, constituting over a third of global vegetable oil production. Currently there is little understanding of the oil palm microbiome and its contribution to plant health and productivity, with existing knowledge based almost entirely on culture dependent studies. We investigated the diversity and composition of the oil palm fungal microbiome in the bulk soil, rhizosphere soil, and roots of 2-, 18-, and 35-year old plantations in Selangor, Malaysia. The fungal community showed substantial variation between the plantations, accounting for 19.7% of community composition, with compartment (root, rhizosphere soil, and bulk soil), and soil properties (pH, C, N, and P) contributing 6.5 and 7.2% of community variation, respectively. Rhizosphere soil and roots supported distinct communities compared to the bulk soil, with significant enrichment of Agaricomycetes, Glomeromycetes, and Lecanoromycetes in roots. Several putative plant pathogens were abundant in roots in all the plantations, including taxa related to Prospodicola mexicana and Pleurostoma sp. The mycorrhizal status and dependency of oil palm has yet to be established, and using 18S rRNA primers we found considerable between-site variation in Glomeromycotinian community composition, accounting for 31.2% of variation. There was evidence for the selection of Glomeromycotinian communities in oil palm roots in the older plantations but compartment had a weak effect on community composition, accounting for 3.9% of variation, while soil variables accounted for 9% of community variation. While diverse Mucoromycotinian fungi were detected, they showed very low abundance and diversity within roots compared to bulk soil, and were not closely related to taxa which have been linked to fine root endophyte mycorrhizal morphology. Many of the fungal sequences showed low similarity to established genera, indicating the presence of substantial novel diversity with significance for plant health within the oil palm microbiome.
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DOI：
10.1111/nph.14990
发表时间：
2018-12
期刊：
The New phytologist
影响因子：
--
作者：
Barnes CJ;van der Gast CJ;McNamara NP;Rowe R;Bending GD
通讯作者：
Bending GD
影响因子：
3.3
作者：
Berruti, Andrea;Desiro, Alessandro;Bonfante, Paola
通讯作者：
Bonfante, Paola
DOI：
10.1007/s10295-015-1724-4
发表时间：
2016-04-01
影响因子：
3.4
作者：
Azura, A. B. Nur;Yusoff, M.;Vikineswary, S.
通讯作者：
Vikineswary, S.
影响因子：
4.9
作者：
Fisher, JB;Jayachandran, K
通讯作者：
Jayachandran, K
影响因子：
9.4
作者：
Albornoz, Felipe E.;Ryan, Megan H.;Standish, Rachel J.
通讯作者：
Standish, Rachel J.