Decreased seizure threshold in an eclampsia-like model induced in pregnant rats with lipopolysaccharide and pentylenetetrazol treatments.
Decreased seizure threshold in an eclampsia-like model induced in pregnant rats with lipopolysaccharide and pentylenetetrazol treatments.
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脂多糖和戊四唑治疗可降低妊娠大鼠子痫样模型中的癫痫阈值
DOI：
10.1371/journal.pone.0089333
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Huang Q;Liu L;Hu B;Di X;Brennecke SP;Liu H
目的子痫是一种鲜为人知但可能致命的妊娠并发症。迄今为止，由于缺乏合适的动物模型，对这种疾病的研究一直受到阻碍。为了纠正这一缺陷，本报告描述了在先前建立的大鼠子痫前期模型中使用戊四唑（PTZ）产生大鼠子痫样模型。方法妊娠第14天尾静脉注射脂多糖1.0µg/kg，建立子痫前期（PE）。PE和对照大鼠（未妊娠，NP；正常妊娠，P）腹腔注射PTZ （40 mg/kg）诱导癫痫发作。在单独实验中，在PTZ前给PE大鼠注射MgSO4 (270 mg/kg IP)，观察其对PTZ诱导癫痫发作的影响。结果与对照组相比，LPS给药后大鼠血压（P<0.01）、尿白蛋白排泄（P<0.05）、血清sFlt-1升高（P<0.05）、PlGF水平降低（P<0.05），并出现肝功能障碍。PTZ成功地诱导了所有实验组的癫痫发作活动。PE-PTZ组癫痫发作潜伏期（73.2±6.6秒）明显低于ptz组（107.0±7.4秒）。MgSO4预处理延长了癫痫发作潜伏期（P<0.05），缩短了癫痫发作时间，降低了癫痫发作率。PE组和PE- ptz组血清炎症因子TNF-α和IL-1β水平显著升高（P<0.05）， MgSO4组血清炎症因子TNF-α和IL-1β水平显著降低（P<0.05）。结论ptz诱导的子痫样大鼠模型与人子痫相似，可为子痫的进一步研究提供有益的工具。子痫前期炎症细胞因子的增加与ptz诱发的癫痫发作阈值的降低是一致的，这表明炎症机制可能有助于癫痫发作活动的易感性，炎症可能在子痫中起重要作用。
Objective Eclampsia is a poorly understood but potentially fatal complication of pregnancy. Research to date on this disorder has been hampered by the lack of a suitable animal model. To correct this deficiency, this report describes the generation of a rat eclampsia-like model using pentylenetetrazol (PTZ) in a previously established rat preeclampsia model. Method Rats were administered lipopolysaccharide (1.0 µg/kg) by tail vein injection on gestational day 14 to establish preeclampsia (PE). PE and control rats (non-pregnant, NP; normal-pregnant, P) were injected intraperitoneally (i.p.) with PTZ (40 mg/kg) to induce seizures. In separate experiments, MgSO4 (270 mg/kg IP) was injected in advance of PTZ into PE rats to observe its effect on PTZ-induced seizures. Results PE conditions were verified in rats after LPS administration by significantly higher blood pressure (P<0.01) and urinary albumin excretion (P<0.05), elevated sFlt-1 (P<0.05) and decreased PlGF serum levels (P<0.05), and evidence of hepatic dysfunction compared to control groups. PTZ successfully induced seizure activity in all groups studied. Latency to seizure was significantly (P<0.01) less in the PE-PTZ group (73.2±6.6 sec.) than in PTZ-treated controls (107.0±7.4 sec.). Pretreatment with MgSO4 prolonged (P<0.05) latency to seizure, shortened seizure duration and decreased seizure rates. Significant increased (P<0.05) in the serum levels of the inflammatory cytokines TNF-α and IL-1β in PE and PE-PTZ groups, and decreased (P<0.05) in their levels following MgSO4 administration. Conclusion This PTZ-induced eclampsia-like rat model is comparable to the human condition of eclampsia and may serve as a useful research tool for future studies of this disease. The increased inflammatory cytokines in preeclampsia are coincident with a decreased threshold for PTZ-induced seizures, suggesting that an inflammatory mechanism may contribute to the susceptibility to seizure activity and inflammation might have an important role in eclampsia.
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影响因子：
5.3
作者：
Cipolla, Marilyn J.;Pusic, Aya D.;Grinberg, Yelena Y.;Chapman, Abbie C.;Poynter, Matthew E.;Kraig, Richard P.
通讯作者：
Kraig, Richard P.
影响因子：
4.3
作者：
De Vivo, Antonio;Baviera, Giovanni;D'Anna, Rosario
通讯作者：
D'Anna, Rosario
影响因子：
2.6
作者：
Alanbay, Ibrahim;Coksuer, Hakan;Baser, Iskender
通讯作者：
Baser, Iskender
影响因子：
2.3
作者：
Amash, Alaa;Holcberg, Gershon;Huleihel, Mahmoud
通讯作者：
Huleihel, Mahmoud
DOI：
10.1016/s0002-9378(12)90855-8
发表时间：
1993-03-01
影响因子：
9.8
作者：
COTTON, DB;HALLAK, M;BERMAN, RF
通讯作者：
BERMAN, RF