Overexpression of stathmin plays a pivotal role in the metastasis of esophageal squamous cell carcinoma.
Overexpression of stathmin plays a pivotal role in the metastasis of esophageal squamous cell carcinoma.
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Stathmin过表达在食管鳞癌转移中发挥关键作用
DOI：
10.18632/oncotarget.18687
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发表时间：
2017-09-22
期刊：
影响因子：
--
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Han G;Wu Z;Zhao N;Zhou L;Liu F;Niu F;Xu Y;Zhao X
目的食管鳞状细胞癌（Esophageal squamous cell carcinoma，ESCC）是严重危害人类健康的恶性肿瘤.分析血清Stathmin水平与ESCC的相关性，阐明Stathmin促进ESCC细胞侵袭和转移的分子机制。方法采用酶联免疫吸附法（ELISA）检测食管鳞癌患者和健康对照者血清Stathmin水平，并对临床参数进行分析。我们建立了stathmin过表达或敲低的ESCC细胞，然后评估stathmin对ESCC侵袭和转移的影响。用人类转录组芯片分析差异表达基因，并用RT-PCR证实。免疫印迹法检测整合素家族、粘着斑激酶（FAK）和细胞外信号调节激酶（ERK）的表达水平。结果食管鳞癌患者血清stathmin水平明显高于正常对照组，且与淋巴结转移、肿瘤分期、肿瘤大小有关。此外，我们发现stathmin在体外和体内促进ESCC细胞的迁移和侵袭。此外，我们证实了整合素α5β1/FAK/ERK通路的激活在stathmin过表达细胞中增加，并通过增强细胞粘附能力来加速细胞运动。结论Stathmin可能是预测食管鳞癌转移的一个潜在生物标志物。
Purpose Esophageal squamous cell carcinoma (ESCC) is a serious malignant tumor that affects human health. We analyzed the correlation between serum stathmin level and ESCC and elucidated the molecular mechanisms of stathmin's promotion of ESCC cell invasion and metastasis. Methods Stathmin level in ESCC and healthy control serum were detected by enzyme-linked immunosorbent assay (ELISA), and the clinical parameters were analyzed. We established ESCC cells with stathmin overexpression or knockdown and then evaluated the effects of stathmin on invasion and metastasis in ESCC. Differentially expressed genes were analyzed by Human Transcriptome Array and confirmed by RT-PCR. The expression levels of the integrin family, focal adhesion kinase (FAK) and extracellular signal-regulated kinase (ERK) were detected by immunoblotting. Results Serum levels of stathmin were significantly higher in ESCC than in control serum and associated with lymph node metastasis, tumor stage and size. Furthermore, we found that stathmin promoted migration and invasion of ESCC cells in vitro and in vivo. In addition, we confirmed that the activation of the integrinα5β1/FAK/ERK pathway is increased in stathmin-overexpression cells and accelerates cell motility by enhancing cell adhesion ability. Conclusion Stathmin may predict a potential metastasis biomarker for ESCC.
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影响因子：
--
作者：
Lin CH;Lin HH;Kuo CY;Kao SH
通讯作者：
Kao SH
影响因子：
16.6
作者：
Mana, Giulia;Clapero, Fabiana;Panieri, Emiliano;Panero, Valentina;Boettcher, Ralph T.;Tseng, Hui-Yuan;Saltarin, Federico;Astanina, Elena;Wolanska, Katarzyna I.;Morgan, Mark R.;Humphries, Martin J.;Santoro, Massimo M.;Serini, Guido;Valdembri, Donatella
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Valdembri, Donatella
影响因子：
5.2
作者：
Dia, Vermont P.;de Mejia, Elvira Gonzalez
通讯作者：
de Mejia, Elvira Gonzalez
影响因子：
8
作者：
Chen, J.;Abi-Daoud, M.;Tron, V. A.
通讯作者：
Tron, V. A.
Stathmin的表达和磷酸化与食管鳞癌细胞迁移相关
影响因子：
4.2
作者：
Liu F;Sun YL;Xu Y;Liu F;Wang LS;Zhao XH
通讯作者：
Zhao XH