Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma.
Expression and phosphorylation of stathmin correlate with cell migration in esophageal squamous cell carcinoma.
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Stathmin的表达和磷酸化与食管鳞癌细胞迁移相关
DOI：
10.3892/or.2012.2157
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发表时间：
2013-02
期刊：
影响因子：
4.2
通讯作者：
Zhao XH
中科院分区：
文献类型：
--
作者：
Liu F;Sun YL;Xu Y;Liu F;Wang LS;Zhao XH
微管在细胞运动等细胞过程中起着广泛的作用。Stathmin是一种重要的微管不稳定蛋白。Stathmin的基本功能与其磷酸化状态密切相关。Stathmin在许多人类癌症中过表达，并且与临床特征如分级、肿瘤大小和预后具有显著关系。我们使用2-DE和免疫组织化学分析证明Stathmin在ESCC组织中过表达。此外，stathmin的过度表达与ESCC的组织学分级显著相关。而与年龄、性别、淋巴结转移无关。用siRNA敲低stathmin损害KYSE 30和KYSE 410细胞中的细胞迁移。当用紫杉醇处理EC 0156细胞时，stathmin被稳定磷酸化并且迁移受损。这些观察结果表明，stathmin可能有更重要的功能，在食管鳞癌的发展和迁移。本研究提供了进一步了解stathmin在ESCC治疗或诊断中的重要性。
Microtubules play extensive roles in cellular processes, including cell motility. Stathmin is an important protein which destabilizes microtubules. The essential function of stathmin is closely associated with its phosphorylation status. Stathmin is overexpressed in many human cancers and has a significant relationship with clinical characteristics such as grade, tumor size and prognosis. We demonstrated that stathmin was overexpressed in ESCC tissues using both 2-DE and immunohistochemistry analysis. In addition, overexpression of stathmin was significantly correlated with histological grade in ESCC. However, no correlation was found with age, gender and lymph node metastasis. Knockdown of stathmin with siRNA impaired cell migration in KYSE30 and KYSE410 cells. When EC0156 cells were treated with paclitaxel, stathmin was stably phosphorylated and migration was impaired. These observations suggest that stathmin may have a more important function in ESCC development and migration. The present study provides further understanding of the importance of stathmin in ESCC therapy or diagnosis.
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影响因子：
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