Depth-dependent detection of microampere currents delivered to monkey V1.
Depth-dependent detection of microampere currents delivered to monkey V1.
复制标题
DOI：
10.1111/j.1460-9568.2009.06695.x
复制
发表时间：
2009-04
期刊：
影响因子：
--
通讯作者：
Slocum WM
中科院分区：
文献类型：
--
作者：
Tehovnik EJ;Slocum WM
猴子可以探测到传递到纹状皮层（V1区）的电刺激。我们研究了这种刺激是否依赖于层位。当恒河猴盯着一个光点时，研究人员要求它检测到100毫秒的电刺激序列，这些电刺激传递到V1区域内的一个部位。一只猴子通过按下一个杠杆来发出刺激的信号，之后他会得到一滴苹果汁作为奖励。对照试验是交错进行的，在这段时间里，没有刺激，猴子没有按下杠杆就会得到奖励。采用0.2 ms阴极优先双相脉冲，以200 Hz的频率输出电流一般小于30 μA。在一些实验中，脉冲持续时间从0.05到0.7 ms不等，并使用阳极优先脉冲。当刺激到V1的最深层（皮质表面以下1.0 ~ 2.5 mm）时，检测到阴极优先脉冲的电流阈值最低（< 10 μA），占50%。此外，在这些深度处，检测刺激的最短时间（< 0.2 ms）和最短潜伏期（< 200 ms）也被观察到。最后，阳极优先脉冲在V1浅层唤起检测响应最有效，阴极优先脉冲在V1深部（皮质表面以下1.75 mm）唤起检测响应最有效。因此，V1的最深层是对电刺激感应最敏感的。
Monkeys can detect electrical stimulation delivered to striate cortex (area V1). We examined whether such stimulation is layer dependent. While remaining fixated on a spot of light, a rhesus monkey was required to detect a 100 ms train of electrical stimulation delivered to a site within area V1. A monkey signaled the delivery of stimulation by depressing a lever after which he was rewarded with a drop of apple juice. Control trials were interleaved during which time no stimulation was delivered and the monkey was rewarded for not depressing the lever. Biphasic pulses were delivered at 200 Hz and the current was typically at or less than 30 μA using 0.2 ms cathode-first biphasic pulses. For some experiments, the pulse duration was varied from 0.05 to 0.7 ms and anode-first pulses were used. The current threshold for detecting cathode-first pulses 50% of the time was the lowest (< 10 μA) when stimulation was delivered to the deepest layers of V1 (between 1.0 and 2.5 mm below the cortical surface). Also the shortest chronaxies (< 0.2 ms) and the shortest latencies (< 200 ms) for detecting the stimulation were observed at these depths. Finally, anode-first pulses were most effective at evoking a detection response in superficial V1 and cathode-first pulses were most effective at evoking a detection response in deep V1 (> 1.75 mm below the cortical surface). Accordingly, the deepest layers of V1 are the most sensitive for the induction of a detection response to electrical stimulation in monkeys.
登录
查看更多内容
影响因子：
3.7
作者：
Felleman, Daniel J.;Van Essen, David C.
通讯作者：
Van Essen, David C.
影响因子：
5.5
作者：
DOBELLE, WH;MLADEJOVSKY, MG
通讯作者：
MLADEJOVSKY, MG
影响因子：
2.5
作者：
DeYoe, EA;Lewine, JD;Doty, RW
通讯作者：
Doty, RW
影响因子：
24.8
作者：
Doty, R W
通讯作者：
Doty, R W
影响因子：
2.5
作者：
DOTY, RW;RUTLEDGE, LT
通讯作者：
RUTLEDGE, LT