Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood.
Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood.
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DOI：
10.1038/nbt.1507
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发表时间：
2008-12
影响因子：
46.9
通讯作者：
Heath, James R.
中科院分区：
文献类型：
--
作者：
Fan, Rong;Vermesh, Ophir;Srivastava, Alok;Yen, Brian K. H.;Qin, Lidong;Ahmad, Habib;Kwong, Gabriel A.;Liu, Chao-Chao;Gould, Juliane;Hood, Leroy;Heath, James R.
血液构成了人类蛋白质组的最大版本。血浆蛋白质谱的变化可以反映与许多人类疾病相关的生理或病理状况，使血液成为临床诊断的最重要流体。然而，只有少数血浆蛋白用于常规临床试验。这是由于许多原因，包括血浆蛋白质组的内在复杂性、人类疾病的异质性以及与采样血液中蛋白质降解相关的快速动力学。简单的技术，可以灵敏地采样大量的蛋白质在广泛的浓度范围内，从少量的血液，并在几分钟内的样品收集，将有助于解决这些问题。在此，我们报告了一个集成的微流控系统，称为集成血液条形码芯片（IBBC）。它能够实现芯片上血液分离，并从少量血液样本（包括全血的手指针刺）中快速测量一组血浆蛋白。该平台具有廉价，非侵入性和信息丰富的临床诊断的潜力，特别是对于即时护理。
Blood comprises the largest version of the human proteome. Changes of plasma protein profiles can reflect physiological or pathological conditions associated with many human diseases, making blood the most important fluid for clinical diagnostics. Nevertheless, only a handful of plasma proteins are utilized in routine clinical tests. This is due to a host of reasons, including the intrinsic complexity of the plasma proteome, the heterogeneity of human diseases and the fast kinetics associated with protein degradation in sampled blood. Simple technologies that can sensitively sample large numbers of proteins over broad concentration ranges, from small amounts of blood, and within minutes of sample collection, would assist in solving these problems. Herein, we report on an integrated microfluidic system, called the Integrated Blood Barcode Chip (IBBC). It enables on-chip blood separation and the rapid measurement of a panel of plasma proteins from small quantities of blood samples including a fingerprick of whole blood. This platform holds potential for inexpensive, non-invasive, and informative clinical diagnoses, particularly, for point-of-care.
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