Regulation of a High-Iron Diet on Lipid Metabolism and Gut Microbiota in Mice.
Regulation of a High-Iron Diet on Lipid Metabolism and Gut Microbiota in Mice.
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高铁饮食对小鼠脂质代谢和肠道微生物群的调节
DOI：
10.3390/ani12162063
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发表时间：
2022-08-13
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影响因子：
--
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中科院分区：
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铁是一种必需的微量营养素，参与许多重要的生理活动，对动物的生长发育起着关键作用。然而，暴露于高水平的膳食铁会对动物的生长性能、脂肪代谢和肠道微生物区系产生影响。在这项研究中，我们评估了高铁饮食对小鼠脂肪代谢和肠道微生物区系的影响。我们的研究表明，高铁饮食通过下调小鼠肝脏和脂肪组织中的脂肪生成和上调脂肪分解来减少脂肪积累和脂肪沉积。此外，高铁饮食可引起十二指肠炎症的轻微肠道损伤，并重塑肠道微生物区系，但肠道微生物区系在调节脂质代谢中的作用有待进一步探讨。因此，本研究表明，高铁饮食会对小鼠健康产生负面影响，建议严格控制畜禽的铁摄入量。铁稳态紊乱与脂代谢失衡有关，但具体相互作用尚不清楚。在本研究中，我们研究了高铁饮食对小鼠脂代谢的影响。以普通饲料(WT)和高铁饲料(WT+Fe)喂养C57BL/6小鼠12周。我们发现，与WT组小鼠相比，WT+Fe组小鼠的体重增加、体脂和肝脏脂肪积聚明显减少。因此，高铁饮食的小鼠血清总胆固醇和甘油三酯水平都降低了。此外，WT+Fe组小鼠肝脏和脂肪组织中调控脂肪生成和脂肪细胞分化的基因表达显著减少，而脂肪水解酶基因的表达显著增加，这与其显著缩小脂肪细胞的大小一致。此外，高铁饮食降低了有益细菌(阿克曼西亚、双歧杆菌和乳杆菌)的相对丰度，增加了病原菌(龙伯氏菌和白纹伊氏杆菌)的相对丰度。因此，我们的研究表明，高铁饮食通过抑制脂肪生成和促进脂肪分解来减少脂肪沉积。高铁饮食引起的肠道微生物组成改变可能在调节脂肪代谢方面起不到关键作用，但可能会导致肠道炎症和肠道宿主-微生物界面绒毛形态受损等不良副作用。这些发现为铁、脂肪代谢和肠道微生物区系之间的关系提供了新的见解。
Iron is an essential micronutrient involved in many important physiological activities and plays a key role in growth and development of animals. However, exposure to high levels of dietary iron has an effect on growth performance, lipid metabolism, and gut microbiota in animals. In this study, we evaluated the effects of a high-iron diet on lipid metabolism and gut microbiota in mice. Our study shows that a high-iron diet decreased fat accumulation and lipid deposition by downregulating adipogenesis and upregulating lipolysis in the liver and adipose tissues of mice. In addition, a high-iron diet induced slight intestinal damage with duodenal inflammation, and reshaped the gut microbiota, but the role of gut microbiota in regulating lipid metabolism needs to be further explored. Hence, this study shows that a high-iron diet can negatively affect mice health, and it is recommended to strictly control the intake of iron in livestock and poultry. Iron homeostasis disorder is associated with the imbalance of lipid metabolism, while the specific interaction remains unclear. In the present study, we investigated the effect of a high-iron diet on lipid metabolism in mice. The C57BL/6 mice were fed with a normal diet (WT) or a high-iron diet (WT + Fe) for 12 weeks. We found that mice in the WT + Fe group showed a significant decrease in body weight gain, body fat and lipid accumulation of liver when compared with mice in the WT group. Accordingly, serum total cholesterol and triglyceride levels were both reduced in mice with a high-iron diet. Moreover, mice in the WT + Fe group exhibited a significant decrease in expression of genes regulating adipogenesis and adipocyte differentiation, and a significant increase in expression of fat hydrolysis enzyme genes in both liver and adipose tissues, which was consistent with their dramatic reduction in adipocyte cell size. In addition, a high-iron diet decreased the relative abundance of beneficial bacteria (Akkermansia, Bifidobacterium and Lactobacillus) and increased the relative abundance of pathogenic bacteria (Romboutsia and Erysipelatoclostridium). Thus, our research revealed that a high-iron diet reduced lipid deposition by inhibiting adipogenesis and promoting lipolysis. Altered gut microbial composition induced by a high-iron diet may not play a critical role in regulating lipid metabolism, but might cause unwanted side effects such as intestinal inflammation and damaged villi morphology at the intestinal host–microbe interface. These findings provide new insights into the relationship among iron, lipid metabolism and gut microbiota.
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影响因子：
2.7
作者：
Kell DB
通讯作者：
Kell DB
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
影响因子：
3.9
作者：
Cerami C
通讯作者：
Cerami C
影响因子：
3.4
作者：
Burke KE;Lamont JT
通讯作者：
Lamont JT