MSG is involved in sperm gelatinolytic activity in the prawn, Macrobrachium rosenbergii
MSG is involved in sperm gelatinolytic activity in the prawn, Macrobrachium rosenbergii
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味精参与罗氏沼虾的精子明胶分解活性
DOI：
10.1007/s11434-012-5597-x
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发表时间：
2013-01
影响因子：
--
通讯作者：
Yang WeiJun
中科院分区：
文献类型：
--
作者：
Yang Fan;Qian YeQing;Ma WenMing;Li Ye;Yang JinShu;Yang WeiJun
男性生殖道蛋白酶被认为在受精过程中起着关键作用。与哺乳动物相比，关于甲壳类动物精子蛋白酶的数据有限。我们之前从罗氏巨虾（Macrobrachiumrosenbergii）中发现了一种新的精子明胶酶（MSG），它被一种与雄性生殖相关的kazal型蛋白酶抑制剂（MRPINK）特异性抑制。本研究通过Western blot发现，味精分布于输精管和壶腹末端。免疫组化分析显示，味精在分泌性上皮细胞中表达，精子主要分布在基区。RNA干扰（RNAi）介导的味精敲除导致精子明胶溶解活性的显著丧失。综上所述，结果表明，味精与受精过程密切相关，并可能参与了受精过程。
Proteases in male reproductive tract are considered to play key roles in fertilization processes. In contrast to mammals, there are limited data concerning crustacean sperm proteases. We previously identified a novel sperm gelatinase (MSG) from Macrobrachiumrosenbergii that was inhibited by a male reproduction-related Kazal-type protease inhibitor (MRPINK) specifically. In the present study, MSG was found to be distributed on the vas deferens and terminal ampullae by Western blot. Immunohistochemical analyses revealed that MSG was expressed in secretory epithelial cells and sperm distributed mainly at the base zone. RNA interference (RNAi) mediated knock-down of MSG resulted in a marked loss of sperm gelatinolytic activity. Taken together, the results show that MSG is closely linked to and probably involved in the fertilization process.
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影响因子：
6.7
作者：
P. Uhrin;C. Schöfer;J. Zaujec;L. Ryban;Mario Hilpert;K. Weipoltshammer;I. Jerabek;Irene Pirtzkall;Margareta Furtmüller;M. Dewerchin;B. Binder;M. Geiger
通讯作者：
P. Uhrin;C. Schöfer;J. Zaujec;L. Ryban;Mario Hilpert;K. Weipoltshammer;I. Jerabek;Irene Pirtzkall;Margareta Furtmüller;M. Dewerchin;B. Binder;M. Geiger
DOI：
10.1016/j.bbrc.2005.12.186
发表时间：
2006-03-10
影响因子：
3.1
作者：
Yodmuang, S;Tirasophon, W;Panyim, S
通讯作者：
Panyim, S
影响因子：
4.8
作者：
Honda, A;Yamagata, K;Baba, T
通讯作者：
Baba, T
DOI：
10.1074/jbc.273.17.10470
发表时间：
1998-04
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
K. Yamagata;Keitaro Murayama;M. Okabe;K. Toshimori;T. Nakanishi;S. Kashiwabara;T. Baba
通讯作者：
K. Yamagata;Keitaro Murayama;M. Okabe;K. Toshimori;T. Nakanishi;S. Kashiwabara;T. Baba
DOI：
10.1111/j.1439-0531.2007.00977.x
发表时间：
2008-12
期刊：
Reproduction in domestic animals = Zuchthygiene
影响因子：
--
作者：
M. Deppe;P. Morales;R. Sánchez
通讯作者：
M. Deppe;P. Morales;R. Sánchez