Six novel Y chromosome genes in Anopheles mosquitoes discovered by independently sequencing males and females.
Six novel Y chromosome genes in Anopheles mosquitoes discovered by independently sequencing males and females.
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DOI：
10.1186/1471-2164-14-273
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发表时间：
2013-04-23
期刊：
影响因子：
4.4
通讯作者：
Tu Z
中科院分区：
文献类型：
--
作者：
Hall AB;Qi Y;Timoshevskiy V;Sharakhova MV;Sharakhov IV;Tu Z
Y 染色体负责不同物种雄性发育、雄性生育力和其他雄性相关功能的启动。然而，由于研究富含重复的 Y 基因非常艰巨，除少数模式物种外，Y 基因很少被表征。染色体商 (CQ) 是系统发现 Y 染色体基因的一种新方法。在 CQ 方法中，男性和女性的基因组 DNA 被独立测序并与候选参考序列进行比对。与参考序列的比对数量的女性与男性的比率，称为染色体商（CQ）的参数，用于确定序列是否是Y连锁的。使用CQ方法，我们成功地鉴定了智人和果蝇的已知Y序列。 CQ 方法促进了疟疾蚊子 Anopheles stephensi 和 An.Y 染色体序列的发现。冈比亚。通过与blastn的转录组序列数据进行比较，发现了按蚊的6个Y基因，每个物种各有3个。所有六个基因都在早期胚胎中表达。三个中的两个安。史蒂芬西 Y 基因最近是从常染色体或 X 获得的。斯蒂芬西和安.冈比亚亚属属于同一亚属，我们没有发现物种间共享 Y 基因的证据。 CQ 方法可以使用男性和女性序列数据的比对比率可靠地识别 Y 染色体序列。 CQ 方法广泛适用于具有由下一代测序数据产生的片段化基因组组装的物种。对本研究中表征的 6 个 Y 基因的分析表明，An. 和 An. 之间的 Y 染色体快速进化。斯蒂芬西和安.冈比亚。通过 CQ 方法发现的按蚊 Y 基因为种群和系统发育分析提供了独特的标记，并为通过操纵性二态性和生育力的新型蚊子控制措施提供了机会。
Y chromosomes are responsible for the initiation of male development, male fertility, and other male-related functions in diverse species. However, Y genes are rarely characterized outside a few model species due to the arduous nature of studying the repeat-rich Y. The chromosome quotient (CQ) is a novel approach to systematically discover Y chromosome genes. In the CQ method, genomic DNA from males and females is sequenced independently and aligned to candidate reference sequences. The female to male ratio of the number of alignments to a reference sequence, a parameter called the chromosome quotient (CQ), is used to determine whether the sequence is Y-linked. Using the CQ method, we successfully identified known Y sequences from Homo sapiens and Drosophila melanogaster. The CQ method facilitated the discovery of Y chromosome sequences from the malaria mosquitoes Anopheles stephensi and An. gambiae. Comparisons to transcriptome sequence data with blastn led to the discovery of six Anopheles Y genes, three from each species. All six genes are expressed in the early embryo. Two of the three An. stephensi Y genes were recently acquired from the autosomes or the X. Although An. stephensi and An. gambiae belong to the same subgenus, we found no evidence of Y genes shared between the species. The CQ method can reliably identify Y chromosome sequences using the ratio of alignments from male and female sequence data. The CQ method is widely applicable to species with fragmented genome assemblies produced from next-generation sequencing data. Analysis of the six Y genes characterized in this study indicates rapid Y chromosome evolution between An. stephensi and An. gambiae. The Anopheles Y genes discovered by the CQ method provide unique markers for population and phylogenetic analysis, and opportunities for novel mosquito control measures through the manipulation of sexual dimorphism and fertility.
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影响因子：
64.8
作者：
Hughes, Jennifer F.;Skaletsky, Helen;Brown, Laura G.;Pyntikova, Tatyana;Graves, Tina;Fulton, Robert S.;Dugan, Shannon;Ding, Yan;Buhay, Christian J.;Kremitzki, Colin;Wang, Qiaoyan;Shen, Hua;Holder, Michael;Villasana, Donna;Nazareth, Lynne V.;Cree, Andrew;Courtney, Laura;Veizer, Joelle;Kotkiewicz, Holland;Cho, Ting-Jan;Koutseva, Natalia;Rozen, Steve;Muzny, Donna M.;Warren, Wesley C.;Gibbs, Richard A.;Wilson, Richard K.;Page, David C.
通讯作者：
Page, David C.
影响因子：
3.7
作者：
Bargielowski I;Alphey L;Koella JC
通讯作者：
Koella JC
DOI：
10.1098/rstb.2000.0717
发表时间：
2000-11-29
影响因子：
6.3
作者：
Charlesworth, B;Charlesworth, D
通讯作者：
Charlesworth, D
影响因子：
7.4
作者：
Hindson, Benjamin J.;Ness, Kevin D.;Masquelier, Donald A.;Belgrader, Phillip;Heredia, Nicholas J.;Makarewicz, Anthony J.;Bright, Isaac J.;Lucero, Michael Y.;Hiddessen, Amy L.;Legler, Tina C.;Kitano, Tyler K.;Hodel, Michael R.;Petersen, Jonathan F.;Wyatt, Paul W.;Steenblock, Erin R.;Shah, Pallavi H.;Bousse, Luc J.;Troup, Camille B.;Mellen, Jeffrey C.;Wittmann, Dean K.;Erndt, Nicholas G.;Cauley, Thomas H.;Koehler, Ryan T.;So, Austin P.;Dube, Simant;Rose, Klint A.;Montesclaros, Luz;Wang, Shenglong;Stumbo, David P.;Hodges, Shawn P.;Romine, Steven;Milanovich, Fred P.;White, Helen E.;Regan, John F.;Karlin-Neumann, George A.;Hindson, Christopher M.;Saxonov, Serge;Colston, Bill W.
通讯作者：
Colston, Bill W.
DOI：
10.1073/pnas.231484998
发表时间：
2001-11-06
影响因子：
11.1
作者：
Carvalho, AB;Dobo, BA;Clark, AG
通讯作者：
Clark, AG