Cost of mating and insemination capacity of a genetically modified mosquito Aedes aegypti OX513A compared to its wild type counterpart.
Cost of mating and insemination capacity of a genetically modified mosquito Aedes aegypti OX513A compared to its wild type counterpart.
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DOI：
10.1371/journal.pone.0026086
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Koella JC
中科院分区：
文献类型：
--
作者：
Bargielowski I;Alphey L;Koella JC
以传统昆虫控制技术为模型，实施基于遗传学的昆虫控制策略的想法正变得越来越流行。在本文中，我们比较了携带四环素抑制的致命正反馈系统（OX513A）的转基因埃及伊蚊系与野生型埃及伊蚊的授精能力和求爱和交配成本。转基因雄性在其一生中与野生型雄性交配的数量仅为雌性的一半多一点。在交配前提供几天的休息时间，对两系雄性授精的雌性总数没有显著影响，但确实增加了它们的寿命。就能量投入而言，产生精子的成本较低；将精子转移到接受交配的雌性身上的成本要高得多。与难缠的雌性继续交配表明，雄性在投入大量精力求爱之前无法识别难缠的雌性。尽管在一生中，OX513A雄性与雌性的授精次数较少，但前三天授精的雌性数量在两个系的雄性之间是相似的，这表明RIDL的确定成本可能对基于sitd的控制计划的结果影响不大，该计划经常释放转基因雄性。
The idea of implementing genetics-based insect control strategies modelled on the traditional SIT is becoming increasingly popular. In this paper we compare a genetically modified line of Aedes aegypti carrying a tetracycline repressible, lethal positive feedback system (OX513A) with its wild type counterpart with respect to their insemination capacities and the cost of courtship and mating. Genetically modified males inseminated just over half as many females as the wild type males during their lifetime. Providing days of rest from mating had no significant effect on the total number of females inseminated by males of either line, but it did increase their longevity. Producing sperm had a low cost in terms of energy investment; the cost of transferring this sperm to a receptive female was much higher. Continued mating attempts with refractory females suggest that males could not identify refractory females before investing substantial energy in courtship. Although over a lifetime OX513A males inseminated fewer females, the number of females inseminated over the first three days, was similar between males of the two lines, suggesting that the identified cost of RIDL may have little impact on the outcome of SIT-based control programmes with frequent releases of the genetically modified males.
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影响因子：
5.4
作者：
Phuc HK;Andreasen MH;Burton RS;Vass C;Epton MJ;Pape G;Fu G;Condon KC;Scaife S;Donnelly CA;Coleman PG;White-Cooper H;Alphey L
通讯作者：
Alphey L
DOI：
10.1073/pnas.1000251107
发表时间：
2010-03-09
影响因子：
11.1
作者：
Fu, Guoliang;Lees, Rosemary S.;Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
46.9
作者：
Gong, P;Epton, MJ;Alphey, L
通讯作者：
Alphey, L
DOI：
10.1186/jbiol143
发表时间：
2009-05-01
期刊：
Journal of Biology (London)
影响因子：
--
作者：
Alphey, Luke
通讯作者：
Alphey, Luke
影响因子：
2.5
作者：
Gaskin, T;Futerman, P;Chapman, T
通讯作者：
Chapman, T