Biological evaluation of the effect of magnetron sputtered Ca/P coatings on osteoblast-like cells in vitro.
Biological evaluation of the effect of magnetron sputtered Ca/P coatings on osteoblast-like cells in vitro.
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磁控溅射 Ca/P 涂层对体外成骨细胞样细胞影响的生物学评价。
DOI：
10.1002/jbm.820290808
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
J. Jansen
中科院分区：
文献类型：
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作者：
J. Hulshoff;K. van Dijk;J. van der Waerden;J. Wolke;L. Ginsel;J. Jansen
使用大鼠骨髓细胞培养物来评估无定形和结晶薄磷酸钙 (Ca/P) 涂层的成骨潜力。使用射频磁控溅射程序将涂层沉积在钛盘上。非晶态和晶态等离子喷涂 Ca/P 涂层和非涂层钛盘作为参考材料。使用定量（附着和增殖率）和定性（扫描电子显微镜）技术分析细胞行为。各种材料之间的细胞附着和增殖率没有发现显着差异。扫描电子显微镜显示在非晶等离子体喷涂和两种类型的磁控溅射涂层上培养 18 天后细胞外基质形成。此外，没有观察到磁控溅射涂层的严重退化。它们甚至似乎能诱导磷灰石的形成。根据结果，我们得出结论，磁控溅射似乎是制造生物活性陶瓷涂层的一种有前途的方法。
A rat bone marrow cell culture was used to evaluate the osteogenic potential of amorphous and crystalline thin calcium phosphate (Ca/P) coatings. The coatings were deposited on titanium discs using a radiofrequency magnetron sputter procedure. Amorphous and crystalline plasma spray Ca/P coated and noncoated titanium discs served as reference material. The cellular behavior was analyzed with quantitative (attachment and proliferation rates) and qualitative (scanning electron microscopy) techniques. No significant differences were found in cell attachment and proliferation rates between the various materials. Scanning electron microscopy showed extracellular matrix formation after 18 days of culture on amorphous plasma-sprayed and the two types of magnetron sputtered coatings. Furthermore, no severe degradation of the magnetron sputtered coatings was observed. They even appeared to induce apatite formation. On basis of the results, we conclude that magnetron sputtering appears to be a promising method to manufacture bioactive ceramic coatings.
DOI：
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发表时间：
1993
期刊：
The Journal of oral implantology
影响因子：
--
作者：
J. Lian;G. Stein
通讯作者：
J. Lian;G. Stein
骨和骨内膜牙种植体之间界面区域的高压电子显微镜和传统透射电子显微镜。
DOI：
10.1002/jbm.820260409
发表时间：
1992
期刊：
Journal of biomedical materials research
影响因子：
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作者：
Steflik,DE;Sisk,AL;Parr,GR;Hanes,PJ;Lake,F;Song,MJ;Brewer,P;McKinney,RV
通讯作者：
McKinney,RV