Intracellular signaling involved in macrophage adhesion and FBGC formation as mediated by ligand-substrate interaction.

Intracellular signaling involved in macrophage adhesion and FBGC formation as mediated by ligand-substrate interaction.
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细胞内信号传导涉及巨噬细胞粘附和 FBGC 形成,由配体-底物相互作用介导。

DOI:
10.1002/jbm.10317
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发表时间:
2002
期刊:
Journal of biomedical materials research
影响因子:
--
通讯作者:
Liu,Yiping
Liu,Yiping
中科院分区:
--
文献类型:
--
作者:
Kao,WeiyuanJohn;Liu,Yiping

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Fibronectin and RGD‐ and/or PHSRN‐containing oligopeptides were preadsorbed onto physicochemically distinct substrata: polyethyleneglycol‐based networks or tissue culture polystyrene (TCPS). The role of selected signaling kinases (namely protein tyrosine kinases, protein serine/threonine kinases, PI3‐kinase, Src, and MAPK) in the adhesion of human primary blood‐derived macrophages and the formation of foreign‐body giant cells (FBGC) on these modified substrata was investigated. The involvement of individual intracellular signaling molecules in mediating macrophage adhesion dynamically varied with the culture time, substrate, and ligand. For example, fibronectin on TCPS or networks involved similar signaling events for macrophage adhesion; however, fibronectin and G3RGDG6PHSRNG, but not peptides with other RGD and/or PHSRN orientations, mediated similar signaling events for macrophage adhesion on TCPS but mediated different signaling events on networks. Depending on the substrate, a specific molecule (i.e., Src, protein kinase C) within the protein tyrosine kinase or protein serine/threonine kinase family was either an antagonist or agonist in mediating FBGC formation. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 62: 478–487, 2002
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