Virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in Shigella flexneri 2a
Virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in Shigella flexneri 2a
复制标题
福氏志贺氏菌 2a 中包含 230 kb 质粒的 31 kb 的毒力相关遗传区域
DOI：
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发表时间：
1988
影响因子：
3.2
通讯作者：
Masanosuke Yoshikawa
中科院分区：
文献类型：
--
作者：
C. Sasakawa;K. Kamata;Takashi Sakai;Souich Makino;Masatoshi Yamada;Nobuhiko Okada;Masanosuke Yoshikawa
通过随机转座子 Tn5 插入，我们之前在福氏志贺氏菌 2a 的 230 kilobase 质粒 pMYSH6000 中鉴定了 6 个毒力相关的 SalI 片段：B、D、F、G、H 和 P。在本研究中，我们分析了 pMYSH6000 的四个连续 SalI 片段 B、P、H 和 D 上的 134 个独立 Tn5 插入位点，并鉴定了 5 个毒力相关区域；四种与诱导 Sereny 试验阳性 (Ser)、侵入上皮细胞 (Inv)、与刚果红 (Pcr) 结合和抑制细菌生长 (Igr) 有关，一种与 Ser 和 Inv 相关，但与 Pcr 或 Igr 表型无关。杂交研究表明，这些毒力相关的 DNA 区域在四种志贺氏菌和肠侵袭性大肠杆菌的 15 个毒力质粒中高度保守。这些数据表明，志贺氏菌毒力表型的完全表达需要毒力质粒上至少七个独立的遗传决定簇。
By random transposon Tn5 insertions, we previously identified six virulence-associated SalI fragments, B, D, F, G, H, and P, in the 230-kilobase plasmid pMYSH6000 of Shigella flexneri 2a. In this study, we analyzed the sites of 134 independent Tn5 insertions on four contiguous SalI fragments, B, P, H, and D, of pMYSH6000 and identified five virulence-associated regions; four were associated with inducing a positive Sereny test (Ser), invasion into epithelial cells (Inv), binding to Congo red (Pcr), and inhibition of bacterial growth (Igr), and one was associated with the Ser and Inv but not with the Pcr or Igr phenotypes. Hybridization studies revealed that these virulence-associated DNA regions were highly conserved among 15 other virulence plasmids of four species of Shigella and enteroinvasive Escherichia coli. These data indicate that at least seven separate genetic determinants on the virulence plasmid are required for full expression of the virulence phenotype of shigellae.
IS50 介导的逆转座。
影响因子：
5.6
作者：
Sasakawa,C;Berg,DE
通讯作者：
Berg,DE