Characterization of a new bacterium with high alkaline keratinase activity from Calotes versicolor feces
Characterization of a new bacterium with high alkaline keratinase activity from Calotes versicolor feces
复制标题
杂色蜥蜴粪便中具有高碱性角蛋白酶活性的新细菌的表征
作者：
岳晓禹
从221份动物粪便样品中分离到842株产芽孢细菌，经形态学、生化和16 S rDNA鉴定，从变色蜥蜴粪便中分离到1株新的羽毛降解菌，命名为芽孢杆菌（Bacillus sp. 50-3）。该菌完全降解天然羽毛在温和的条件下，在更短的时间（36小时），当鸡毛作为唯一的碳源和氮源。在37 ℃、pH7.0的羽毛粉培养基中，最大酶活为680 ± 25 U/ml，最适反应时间为36 h。芽孢杆菌50-3以羽毛角蛋白为底物的最适酶浓度为1.0%（w/v）。芽孢杆菌50-3对羽毛的高效降解表明其在生物技术，特别是在减少环境污染方面具有潜在的应用前景。
A total of 842 spore-forming bacterial strains were isolated from 221 animal feces samples, in which a new feather-degrading bacterium identified as Bacillus sp. 50-3 was isolated from Calotes versicolor (an agamid lizard) feces based on morphological, biochemical, and 16S rDNA tests. The bacterium degraded native feathers completely at mild conditions and in much shorter time (36 h) when chicken feathers were applied as the sole carbon and nitrogen source. The maximum keratinase activity and the optimum reaction time were 680 ± 25 U/ml and 36 h respectively at 37°C and pH 7.0 in feather meal medium. The optimal keratinase concentration of Bacillus sp. 50-3 with feather keratin as substrate was 1.0 % (w/v). The high efficiency of Bacillus sp. 50-3 in feather degradation suggests its potential applications in biotechnological processes, especially in decreasing environmental pollution.
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DOI：
--
发表时间：
2016-06
期刊：
International journal of applied research
影响因子：
--
作者：
Pradeep Kumar;Shreya Sharma
通讯作者：
Pradeep Kumar;Shreya Sharma
影响因子：
2.8
作者：
Y. Nakada;Y. Ohta
通讯作者：
Y. Nakada;Y. Ohta
影响因子：
11.4
作者：
Onifade, AA;Al-Sane, NA;Al-Zarban, S
通讯作者：
Al-Zarban, S
影响因子：
4.4
作者：
Shih, IL;Wu, PJ;Shieh, CJ
通讯作者：
Shieh, CJ
影响因子：
4.4
作者：
Kim, JM;Lim, WJ;Suh, HJ
通讯作者：
Suh, HJ