HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION OF DIHYDROXYLATED VITAMIN D3 METABOLITES USING MOBILE PHASE CONTAINING CYCLODEXTRIN
HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC SEPARATION OF DIHYDROXYLATED VITAMIN D3 METABOLITES USING MOBILE PHASE CONTAINING CYCLODEXTRIN
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使用含有环糊精的流动相对二羟基维生素 D3 代谢物进行高效液相色谱分离
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
K. Shimada
中科院分区：
文献类型：
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作者：
T. Higashi;A. Ogasawara;K. Shimada
以环糊精（CD）为流动相添加剂，采用包合高效液相色谱法分离侧链二羟基化维生素D3代谢物。以乙腈为有机改性剂，在流动相中加入γ-CD，明确分离了24,25-二羟基维生素D3及其3-外聚体。对25,26-二羟基维生素D3的25-外映体进行了一定程度的分离，流动相分别以甲醇和二甲基化β-CD为有机改性剂和添加剂。这些分离方法应用于25-羟基维生素D3大鼠血浆中代谢物的检测。衍生化后用液相色谱/串联质谱法鉴定了这些代谢物。
The separation of side chain dihydroxylated vitamin D3 metabolites by inclusion high performance liquid chromatography using cyclodextrin (CD) as the mobile phase additive was examined. 24,25-Dihydroxyvitamin D3 and its 3-epimer were clearly separated by the addition of γ-CD to the mobile phase using acetonitrile as the organic modifier. The separation of the 25-epimers of 25,26-dihydroxyvitamin D3 has been done, to a certain extent, with the mobile phase using methanol and dimethylated β-CD as the organic modifier and the additive, respectively. These separation methods were applied to the detection of the metabolites in the plasma of the rat administered 25-hydroxyvitamin D3. These metabolites were also identified by liquid chromatography/tandem mass spectrometry after derivatization.
23S,25-二羟基维生素 D3 作为维生素 D3 的循环代谢物。
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Napoli,JL;Pramanik,BC;Partridge,JJ;Uskoković,MR;Horst,RL
通讯作者：
Horst,RL
23,25-二羟基维生素 D3（维生素 D3 的体内代谢物）的分离和鉴定。
影响因子：
2.9
作者：
Tanaka,Y;Wichmann,JK;Schnoes,HK;DeLuca,HF
通讯作者：
DeLuca,HF
天然 25,26-二羟基维生素 D3 是一种差向异构混合物。
DOI：
10.1073/pnas.80.17.5286
发表时间：
1983
影响因子：
11.1
作者：
Ikekawa,N;Koizumi,N;Ohshima,E;Ishizuka,S;Takeshita,T;Tanaka,Y;DeLuca,HF
通讯作者：
DeLuca,HF