In vivo diagnostic imaging using micro-CT: sequential and comparative evaluation of rodent models for hepatic/brain ischemia and stroke.
In vivo diagnostic imaging using micro-CT: sequential and comparative evaluation of rodent models for hepatic/brain ischemia and stroke.
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使用微型 CT 的体内诊断成像：对肝/脑缺血和中风的啮齿动物模型进行连续和比较评估。
DOI：
10.1371/journal.pone.0032342
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Murakami T
中科院分区：
文献类型：
--
作者：
Hayasaka N;Nagai N;Kawao N;Niwa A;Yoshioka Y;Mori Y;Shigeta H;Kashiwagi N;Miyazawa M;Satou T;Higashino H;Matsuo O;Murakami T
越来越需要动物疾病模型来进行病理生理学研究和有效的药物筛选。然而，有效使用这些模型的技术障碍之一是难以对相同的动物进行非侵入性和顺序监测。Micro-CT是动物模型系列诊断成像的有力工具。然而，与目前CT在临床领域的应用不同，软组织对比分辨率，特别是在大脑中的分辨率，不足以进行详细的分析。我们在这份报告中讨论了软组织对比分辨率的问题。我们在小鼠实验性脑梗塞和肝脏缺血模型上进行了增强CT(CECT)检查。通过测量病变体积或高密度区(%HAA)的比率来量化每个病变的病理变化，以指示血管通透性增加。我们还比较了使用Micro-CT和11.7-T Micro-MRI获得的中风大鼠和脑缺血小鼠的脑图像。组织病理学分析证实CECT的诊断。在脑梗塞模型中，血管通透性在手术后3d至1周内增加，伊文思蓝渗漏也证实了这一点。对肝脏损伤体积和%HAA的测量表明，不同遗传背景的小鼠在恢复过程中存在差异。对同一卒中大鼠或缺血小鼠的CT和MR图像进行比较，结果表明，CT图像的体积测量精度、空间分辨率和对比度分辨率与MRI相当。成像结果也与组织学资料相一致。这项研究表明，CECT扫描方法对啮齿动物包括脑在内的组织/器官的病理损害进行定量和定性评估是有用的，也适用于对相同动物的纵向观察。
There is an increasing need for animal disease models for pathophysiological research and efficient drug screening. However, one of the technical barriers to the effective use of the models is the difficulty of non-invasive and sequential monitoring of the same animals. Micro-CT is a powerful tool for serial diagnostic imaging of animal models. However, soft tissue contrast resolution, particularly in the brain, is insufficient for detailed analysis, unlike the current applications of CT in the clinical arena. We address the soft tissue contrast resolution issue in this report. We performed contrast-enhanced CT (CECT) on mouse models of experimental cerebral infarction and hepatic ischemia. Pathological changes in each lesion were quantified for two weeks by measuring the lesion volume or the ratio of high attenuation area (%HAA), indicative of increased vascular permeability. We also compared brain images of stroke rats and ischemic mice acquired with micro-CT to those acquired with 11.7-T micro-MRI. Histopathological analysis was performed to confirm the diagnosis by CECT. In the models of cerebral infarction, vascular permeability was increased from three days through one week after surgical initiation, which was also confirmed by Evans blue dye leakage. Measurement of volume and %HAA of the liver lesions demonstrated differences in the recovery process between mice with distinct genetic backgrounds. Comparison of CT and MR images acquired from the same stroke rats or ischemic mice indicated that accuracy of volumetric measurement, as well as spatial and contrast resolutions of CT images, was comparable to that obtained with MRI. The imaging results were also consistent with the histological data. This study demonstrates that the CECT scanning method is useful in rodents for both quantitative and qualitative evaluations of pathologic lesions in tissues/organs including the brain, and is also suitable for longitudinal observation of the same animals.
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DOI：
10.1097/00004647-200012000-00008
发表时间：
2000-12-01
影响因子：
6.3
作者：
Kim, GW;Sugawara, T;Chan, PH
通讯作者：
Chan, PH
影响因子：
2.8
作者：
Langheinrich, Alexander Claus;Yeniguen, Mesut;Gerriets, Tibo
通讯作者：
Gerriets, Tibo
影响因子：
3
作者：
de Crespigny, Alex;Bou-Reslan, Hani;D'Arceuil, Helen E.
通讯作者：
D'Arceuil, Helen E.
影响因子：
37.8
作者：
Nagai, N;De Mol, M;Collen, D
通讯作者：
Collen, D
影响因子：
3.5
作者：
Seo, Youngho;Hashimoto, Tomoki;Hasegawa, Bruce H.
通讯作者：
Hasegawa, Bruce H.