Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size.
Preparation and characterization of manganese, cobalt and zinc DNA nanoflowers with tuneable morphology, DNA content and size.
复制标题
DOI：
10.1093/nar/gky630
复制
发表时间：
2018-09-06
影响因子：
14.9
通讯作者：
Brown T
中科院分区：
文献类型：
--
作者：
Baker YR;Chen J;Brown J;El-Sagheer AH;Wiseman P;Johnson E;Goddard P;Brown T
最近报道的DNA纳米花是一类有趣的有机-无机杂化材料，其使用DNA聚合酶制备。DNA纳米花联合收割机结合了无机Mg 2 P2 O 7纳米晶体的高表面积和支架与DNA的靶向特性，同时增加了酶的稳定性和增强的细胞摄取。我们已经研究了通过用Mn 2+、Co 2+或Zn 2+取代Mg 2+来化学改性这些纳米花的无机核心的条件，并表征了所得颗粒。这些具有一系列新颖的纳米结构，保留了其镁对应物的酶稳定性，并且Co2+和Mn 2 + DNA纳米花具有额外的磁性。我们研究控制不同形态、DNA含量、杂交特性和大小的条件。此外，我们表明，DNA纳米花的生产并不局限于DNA聚合酶，聚合酶的选择可以影响构建体内的DNA长度。我们预计，对结构、尺寸和化学的额外控制将增强未来的应用。
Recently reported DNA nanoflowers are an interesting class of organic-inorganic hybrid materials which are prepared using DNA polymerases. DNA nanoflowers combine the high surface area and scaffolding of inorganic Mg2P2O7 nanocrystals with the targeting properties of DNA, whilst adding enzymatic stability and enhanced cellular uptake. We have investigated conditions for chemically modifying the inorganic core of these nanoflowers through substitution of Mg2+ with Mn2+, Co2+ or Zn2+ and have characterized the resulting particles. These have a range of novel nanoarchitectures, retain the enzymatic stability of their magnesium counterparts and the Co2+ and Mn2+ DNA nanoflowers have added magnetic properties. We investigate conditions to control different morphologies, DNA content, hybridization properties, and size. Additionally, we show that DNA nanoflower production is not limited to Ф29 DNA polymerase and that the choice of polymerase can influence the DNA length within the constructs. We anticipate that the added control of structure, size and chemistry will enhance future applications.
登录
查看更多内容
影响因子：
15
作者：
Jin, Kyoungsuk;Park, Jimin;Nam, Ki Tae
通讯作者：
Nam, Ki Tae
DOI：
10.1093/ndt/gfv277
发表时间：
2015-12
期刊：
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
影响因子：
--
作者：
Fishbane SN;Singh AK;Cournoyer SH;Jindal KK;Fanti P;Guss CD;Lin VH;Pratt RD;Gupta A
通讯作者：
Gupta A
影响因子：
3.3
作者：
Doat, A;Pellé, F;Lebugle, A
通讯作者：
Lebugle, A
DOI：
10.1080/10426500490468137
发表时间：
2004-07-01
影响因子：
1.3
作者：
Brouzi, K;Ennaciri, A;Harcharras, M
通讯作者：
Harcharras, M
影响因子：
2.7
作者：
FORSYTH, JB;WILKINSON, C;WANKLYN, BM
通讯作者：
WANKLYN, BM