The formation of intestinal organoids in a hanging drop culture.
The formation of intestinal organoids in a hanging drop culture.
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DOI：
10.1007/s10616-018-0194-8
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发表时间：
2018-06
期刊：
影响因子：
2.2
通讯作者：
Pierzchalska M
中科院分区：
文献类型：
--
作者：
Panek M;Grabacka M;Pierzchalska M
Recently organoids have become widely used in vitro models of many tissue and organs. These type of structures, originated from embryonic or adult mammalian intestines, are called “mini guts”. They organize spontaneously when intestinal crypts or stem cells are embedded in the extracellular matrix proteins preparation scaffold (Matrigel). This approach has some disadvantages, as Matrigel is undefined (the concentrations of growth factors and other biologically active components in it may vary from batch to batch), difficult to handle and expensive. Here we show that the organoids derived from chicken embryo intestine are formed in a hanging drop without embedding, providing an attractive alternative for currently used protocols. Using this technique we obtained compact structures composed of contiguous organoids, which were generally similar to chicken organoids cultured in Matrigel in terms of morphology and expression of intestinal epithelial markers. Due to the simplicity, high reproducibility and throughput capacity of hanging drop technique our model may be applied in various studies concerning the gut biology.
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影响因子：
3.6
作者：
Khalil HA;Lei NY;Brinkley G;Scott A;Wang J;Kar UK;Jabaji ZB;Lewis M;Martín MG;Dunn JC;Stelzner MG
通讯作者：
Stelzner MG
影响因子：
3.8
作者：
Kelm, JM;Timmins, NE;Nielsen, LK
通讯作者：
Nielsen, LK
DOI：
10.1007/7651_2016_15
发表时间：
2019-01-01
期刊：
ORGANOIDS
影响因子：
--
作者：
Pierzchalska, Malgorzata;Panek, Malgorzata;Grabacka, Maja
通讯作者：
Grabacka, Maja
影响因子：
1.2
作者：
Foty, Ramsey
通讯作者：
Foty, Ramsey
影响因子：
64.8
作者：
Sato, Toshiro;van Es, Johan H.;Snippert, Hugo J.;Stange, Daniel E.;Vries, Robert G.;van den Born, Maaike;Barker, Nick;Shroyer, Noah F.;van de Wetering, Marc;Clevers, Hans
通讯作者：
Clevers, Hans