The bovine papillomavirus E5 transforming protein can stimulate the transforming activity of EGF and CSF-1 receptors
The bovine papillomavirus E5 transforming protein can stimulate the transforming activity of EGF and CSF-1 receptors
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牛乳头瘤病毒E5转化蛋白可刺激EGF和CSF-1受体的转化活性
DOI：
10.1016/0092-8674(89)90866-0
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发表时间：
1989
期刊：
影响因子：
64.5
通讯作者：
T. Velu
中科院分区：
文献类型：
--
作者：
Patrick Martin;W. Vass;J. Schiller;D. Lowy;T. Velu
牛乳头瘤病毒E5转化基因编码定位于细胞质膜（包括质膜）的44氨基酸蛋白产物。本文报道了E_5能与人表皮生长因子受体和人脑脊液-1受体协同诱导NIH 3T_3细胞的细胞转化。在受体未被配体刺激的情况下，这种协同作用也发生了，而配体处理后，这种协同作用进一步增强。E5与c-ks或c-src之间未观察到合作。E5和高水平EGF受体之间的合作与抑制受体降解和细胞表面活化受体的持久性有关。乳头瘤病毒（PV）是良性上皮性肿瘤的病原体，与动物和人类的某些上皮恶性肿瘤高度相关（综述于Broker和Botchan，1986）。牛乳头瘤病毒1型（Bpv）是一类Pv的原型，可以有效地诱导某些培养细胞的非生产性致瘤性转化，而人乳头瘤病毒（HPV）往往会导致细胞生长特性的不太明显的改变。因此，BPV已被广泛用作Pv体外研究的模型。
The bovine papillomavlrus E5 transforming gene encodes a 44 amlno acid protein product that is localized to cytoplasmic membranes, including the plasma membrane. We now report that E5 can cooperate with human EGF meptom and with human CSF-1 receptors to induce cellular transformation of NIH 3T3 cells. Cooperation occurred in the absence of receptor stimulatlon by ligand, and It was further augmented by treatment with ligand. Cooperation was not seen between E5 and either c-ks or c-src. The cooperation between E5 and high levels of EGF receptor was associated with inhibition of receptor degradation and persistence of activated rrtceptors on the cell surface. We conclude that E5 may enhance the receptor actlvity vla inhibition of receptor down-modulation.Papillomaviruses (PV) are the etiological agents of benign epithelial tumors and have been highly associated with certain epithelial malignancies of animals and humans (reviewed in Broker and Botchan, 1986). Bovine papillomavirus type 1 (Bpv) is the prototype of a class of Pv that can efficiently induce nonproductive tumorigenic transformation of certain cultured cells, while human papillomaviruses (HPV) tend to cause less pronounced alterations in cell growth properties. BPV has therefore been used extensively as a model for the in vitro study of Pv.
影响因子：
2.9
作者：
J. Schlessinger
通讯作者：
J. Schlessinger
影响因子：
56.9
作者：
DYSON, N;HOWLEY, PM;HARLOW, E
通讯作者：
HARLOW, E
牛乳头瘤病毒/猿猴病毒 40 重组病毒表达的牛乳头瘤病毒基因的高效反式激活和形态转化。
DOI：
10.1073/pnas.85.23.9007
发表时间：
1988
影响因子：
11.1
作者：
Settleman,J;DiMaio,D
通讯作者：
DiMaio,D