CircCA12 Promotes Malignant Process via Sponging miR-1184 and Upregulating RAS Family in Bladder Cancer.
CircCA12 Promotes Malignant Process via Sponging miR-1184 and Upregulating RAS Family in Bladder Cancer.
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CircCA12 通过海绵 miR-1184 和上调膀胱癌中的 RAS 家族促进恶性过程
DOI：
10.3389/fgene.2021.663982
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发表时间：
2021
影响因子：
3.7
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Jiang L;Wang Y;Tang H;Li X;Huang C;Liu Z;Zhou F;Wang X;Li Y
环状RNAs(CircRNAs)是一组非编码的RNAs，介导基因表达的调节和病理反应。然而，CircRNAs在膀胱癌(UBC)中的功能和表达模式仍不清楚。在此，我们研究了CircCA12在UBC发育中的作用。QRT-PCR结果显示，CA12在UBC细胞系和组织中显著上调。采用CCK-8、集落形成和异种移植实验来检测CircCA12对UBC的影响。我们的数据表明，沉默CA12抑制了UBC细胞的增殖和集落形成能力。转染si-CirCA12或sh-CircCA12后，UBC细胞在体内和体外的迁移和转移能力均明显减弱。此外，荧光素酶报告和RIP分析表明，CircCA12通过海绵miRNA与miRNA-1184结合，从而上调RAS家族基因(NRAS、KRAS和HRAS)的表达。综上所述，CircCA12/miRNA-1184/RAS家族被认为是UBC进展过程中的一个调节轴。
Circular RNAs (circRNAs) are a panel of non-coding RNAs that mediate the regulation of gene expression, as well as pathological responses. Nonetheless, the function and expression pattern of circRNAs in urinary bladder cancer (UBC) remain unclear. Herein, we examined the function of circCA12 in UBC development. qRT-PCR results demonstrated remarkable circCA12 upregulation in UBC cell lines, as well as tissues. CCK-8, colony formation, and xenograft assays were employed to determine the effect of circCA12 on UBC. Our data illustrated silencing circCA12 repressed the proliferation along with the colony-formation capability of UBC cells. The migration and metastasis potential of UBC cells were remarkably abated in vivo, as well as in vitro after transfection with si-cirCA12 or sh-circCA12. Moreover, luciferase reporter and RIP assays indicated that circCA12 binds to miRNA-1184 through sponging miRNA, thereby up-regulating the expression of RAS family genes (NRAS, KRAS, and HRAS). In conclusion, the circCA12/miRNA-1184/RAS family was identified as a regulatory axis in UBC progression.
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影响因子：
8
作者：
Selth, L. A.;Das, R.;Tilley, W. D.
通讯作者：
Tilley, W. D.
DOI：
10.1016/j.omto.2020.10.003
发表时间：
2020-12-16
期刊：
Molecular therapy oncolytics
影响因子：
--
作者：
Mirzaei H;Hamblin MR
通讯作者：
Hamblin MR
影响因子：
5.7
作者：
Bockorny B;Rusan M;Chen W;Liao RG;Li Y;Piccioni F;Wang J;Tan L;Thorner AR;Li T;Zhang Y;Miao C;Ovesen T;Shapiro GI;Kwiatkowski DJ;Gray NS;Meyerson M;Hammerman PS;Bass AJ
通讯作者：
Bass AJ
DOI：
10.1038/nrc3106
发表时间：
2011-10-13
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
37.3
作者：
Fan, Liyuan;Cao, Qiang;Li, Baosheng
通讯作者：
Li, Baosheng