Effects of Fecal Microbiota Transplantation on Composition in Mice with CKD.
Effects of Fecal Microbiota Transplantation on Composition in Mice with CKD.
复制标题
粪便微生物群移植对CKD小鼠组成的影响。
DOI：
10.3390/toxins12120741
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发表时间：
2020-11-24
期刊：
影响因子：
4.2
通讯作者：
Koppe L
中科院分区：
文献类型：
--
作者：
Barba C;Soulage CO;Caggiano G;Glorieux G;Fouque D;Koppe L
背景：慢性肾脏病（CKD）是一种肾脏疾病，其特征是尿毒症毒素积累，而降低其浓度的策略有限。大量数据支持肠道微生物群在 CKD 并发症中的关键作用，并且是尿毒症毒素产生的主要来源。在这里，我们探讨了粪便微生物移植（FMT）是否可以减轻 CKD 小鼠的代谢并发症和尿毒症毒素积累。方法：通过含有 0.25%（w/w）腺嘌呤的饮食持续 4 周以化学方式诱导肾衰竭。将小鼠随机分为三组：对照组、CKD 组和 CKD + FMT 组。四周后，CKD 小鼠接受健康小鼠的粪便微生物移植 (FMT) 或磷酸盐缓冲盐水作为对照。移植后三周研究了肠道微生物群结构、尿毒症毒素血浆浓度和代谢特征。结果：随着 α 多样性的增加，我们观察到 FMT 治疗后肠道微生物群紊乱得到显着改善。 FMT 进一步减少了硫酸对甲苯酯的积累并改善了葡萄糖耐量。肾功能没有变化。结论：这些数据表明，FMT 通过对肠道微生物群多样性产生有益影响，限制了肠道甲酚途径产生的尿毒症毒素的积累。需要进一步研究FMT的效率、移植时机和移植前的粪便量，以成为CKD患者的治疗选择。
Background: Chronic kidney disease (CKD) is a renal disorder characterized by the accumulation of uremic toxins with limited strategies to reduce their concentrations. A large amount of data supports the pivotal role of intestinal microbiota in CKD complications and as a major source of uremic toxins production. Here, we explored whether fecal microbiota transplantation (FMT) could be attenuated in metabolic complication and uremic toxin accumulation in mice with CKD. Methods: Kidney failure was chemically induced by a diet containing 0.25% (w/w) of adenine for four weeks. Mice were randomized into three groups: control, CKD and CKD + FMT groups. After four weeks, CKD mice underwent fecal microbiota transplantation (FMT) from healthy mice or phosphate buffered saline as control. The gut microbiota structure, uremic toxins plasmatic concentrations, and metabolic profiles were explored three weeks after transplantation. Results: Associated with the increase of alpha diversity, we observed a noticeable improvement of gut microbiota disturbance, after FMT treatment. FMT further decreased p-cresyl sulfate accumulation and improved glucose tolerance. There was no change in kidney function. Conclusions: These data indicate that FMT limited the accumulation of uremic toxins issued from intestinal cresol pathway by a beneficial effect on gut microbiota diversity. Further studies are needed to investigate the FMT efficiency, the timing and feces amount for the transplantation before, to become a therapeutic option in CKD patients.
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影响因子：
30.3
作者：
Devlin AS;Marcobal A;Dodd D;Nayfach S;Plummer N;Meyer T;Pollard KS;Sonnenburg JL;Fischbach MA
通讯作者：
Fischbach MA
DOI：
10.1126/science.aad3292
发表时间：
2016-01-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Baym M;Stone LK;Kishony R
通讯作者：
Kishony R
影响因子：
24.5
作者：
Knoop KA;McDonald KG;Kulkarni DH;Newberry RD
通讯作者：
Newberry RD
影响因子：
3
作者：
Dawson, Lisa F.;Stabler, Richard A.;Wren, Brendan W.
通讯作者：
Wren, Brendan W.
影响因子：
13.6
作者：
Koppe, Laetitia;Pillon, Nicolas J.;Soulage, Christophe O.
通讯作者：
Soulage, Christophe O.