Lipids and topological rules governing membrane protein assembly.
Lipids and topological rules governing membrane protein assembly.
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DOI：
10.1016/j.bbamcr.2013.12.007
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发表时间：
2014-08
影响因子：
5.1
通讯作者：
Vitrac, Heidi
中科院分区：
文献类型：
--
作者：
Bogdanov, Mikhail;Dowhan, William;Vitrac, Heidi
关键词：
膜蛋白折叠和拓扑发生根据给定的脂质谱进行调整，因为脂质和蛋白质共同进化以遵循一组控制最终蛋白质拓扑组织的相互依赖的规则。跨膜结构域 (TMD) 拓扑结构是通过动态过程确定的，其中新生蛋白中的拓扑信号最初由易位子识别和解释，然后根据正内规则给出给定的脂质谱。净零电荷磷脂磷脂酰乙醇胺和其他中性脂质抑制带负电荷残基的易位电位，有利于带正电荷残基的细胞质保留电位（电荷平衡规则）。这解释了为什么带正电的残基比带负电的残基是更有效的拓扑信号。在膜插入期间或之后TMD方向的动态变化归因于非连续合作和集体脂质-蛋白质电荷相互作用以及蛋白质内的长期相互作用。膜蛋白的双拓扑构象异构体的比例不仅在体内而且在体外随着膜脂组成的变化以剂量响应方式变化，因此由膜脂组成决定。两种相反的 TMD 拓扑之间的切换可以在体内的任一方向发生，也可以在脂质体（称为翻转体）中发生，独立于任何其他细胞因素。 TMD 方向的这种脂质依赖性插入后可逆性表明热力学驱动的过程可以在任何时间和任何细胞膜中发生，由脂质成分的变化驱动。这种受脂质环境影响的蛋白质拓扑组织的动态视图揭示了以前未被认识到的细胞调节和理解由错误折叠蛋白质引起的疾病状态的可能性。本文是题为“蛋白质贩运与分泌”的特刊的一部分。
Membrane protein folding and topogenesis are tuned to a given lipid profile since lipids and proteins have co-evolved to follow a set of interdependent rules governing final protein topological organization. Transmembrane domain (TMD) topology is determined via a dynamic process in which topogenic signals in the nascent protein are recognized and interpreted initially by the translocon followed by a given lipid profile in accordance with the Positive Inside Rule. The net zero charged phospholipid phosphatidylethanolamine and other neutral lipids dampen the translocation potential of negatively charged residues in favor of the cytoplasmic retention potential of positively charged residues (Charge Balance Rule). This explains why positively charged residues are more potent topological signals than negatively charged residues. Dynamic changes in orientation of TMDs during or after membrane insertion are attributed to non-sequential cooperative and collective lipid–protein charge interactions as well as long-term interactions within a protein. The proportion of dual topological conformers of a membrane protein varies in a dose responsive manner with changes in the membrane lipid composition not only in vivo but also in vitro and therefore is determined by the membrane lipid composition. Switching between two opposite TMD topologies can occur in either direction in vivo and also in liposomes (designated as fliposomes) independent of any other cellular factors. Such lipid-dependent post-insertional reversibility of TMD orientation indicates a thermodynamically driven process that can occur at any time and in any cell membrane driven by changes in the lipid composition. This dynamic view of protein topological organization influenced by the lipid environment reveals previously unrecognized possibilities for cellular regulation and understanding of disease states resulting from mis-folded proteins. This article is part of a Special Issue entitled: Protein Trafficking & Secretion.
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DOI：
10.1016/j.bbalip.2012.08.007
发表时间：
2013-03
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Dowhan W
通讯作者：
Dowhan W
影响因子：
4.8
作者：
BOGDANOV, M;DOWHAN, W
通讯作者：
DOWHAN, W
影响因子：
5.4
作者：
Dorobantu, Cristina;Macovei, Alina;Branza-Nichita, Norica
通讯作者：
Branza-Nichita, Norica
影响因子：
3.4
作者：
Dowhan, William;Bogdanov, Mikhail
通讯作者：
Bogdanov, Mikhail
DOI：
10.1016/b978-044453219-0.50003-9
发表时间：
2008-01-01
期刊：
BIOCHEMISTRY OF LIPIDS, LIPOPROTEINS AND MEMBRANES, 5TH EDITION
影响因子：
--
作者：
Dowhan, William;Bogdanov, Mikhail;Mileykovskaya, Eugenia
通讯作者：
Mileykovskaya, Eugenia