Single-nucleus RNA-seq reveals that MBD5, MBD6, and SILENZIO maintain silencing in the vegetative cell of developing pollen.
Single-nucleus RNA-seq reveals that MBD5, MBD6, and SILENZIO maintain silencing in the vegetative cell of developing pollen.
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DOI：
10.1016/j.celrep.2022.111699
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发表时间：
2022-11-22
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
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转座因子（TE）的沉默驱动了许多冗余的转录调控机制的进化。拟南芥MBD5、MBD6和SILENZIO在DNA甲基化下游作为TE阻遏物。在这里，我们通过发育中的雄配子体的单核RNA-seq显示，这些阻遏物对于花粉营养细胞中的TE沉默至关重要，花粉营养细胞是一种对受精重要的伴细胞，其经历染色质解压缩。相反，其他沉默突变体（met1，ddm1，mom1，morc）在所有花粉核类型和体细胞中表现出沉默的丧失。我们发现，被MBD5/6抑制的TE在野生型营养细胞核中获得染色质可及性，尽管保持沉默，这表明DNA压缩的损失使它们对MBD5/6的损失敏感。一致的是，交叉mbd 5/6组蛋白1突变体，其中有解浓缩的染色质在叶中，揭示了MBD 5/6依赖的TE在叶中的去阻遏。因此，MBD5/6和SILENZIO作为沉默系统，在染色质致密化受损时特别重要。Ichino等人开发了一种方法来分析单个拟南芥花粉核沿着其发育轨迹的基因表达。这导致他们发现DNA甲基化阅读器MBD5和MBD6的突变导致花粉营养核中转座子沉默的丧失，可能是因为它们的致敏解压缩染色质状态。
Silencing of transposable elements (TEs) drives the evolution of numerous redundant mechanisms of transcriptional regulation. Arabidopsis MBD5, MBD6, and SILENZIO act as TE repressors downstream of DNA methylation. Here, we show, via single-nucleus RNA-seq of developing male gametophytes, that these repressors are critical for TE silencing in the pollen vegetative cell, a companion cell important for fertilization that undergoes chromatin decompaction. Instead, other silencing mutants (met1, ddm1, mom1, morc) show loss of silencing in all pollen nucleus types and somatic cells. We show that TEs repressed by MBD5/6 gain chromatin accessibility in wild-type vegetative nuclei despite remaining silent, suggesting that loss of DNA compaction makes them sensitive to loss of MBD5/6. Consistently, crossing mbd5/6 to histone 1 mutants, which have decondensed chromatin in leaves, reveals derepression of MBD5/6-dependent TEs in leaves. MBD5/6 and SILENZIO thus act as a silencing system particularly important when chromatin compaction is compromised. Ichino et al. develop a method to profile gene expression of individual Arabidopsis pollen nuclei along their developmental trajectories. This leads them to discover that mutation of DNA methylation readers MBD5 and MBD6 causes loss of transposon silencing in pollen vegetative nuclei, likely because of their sensitized decompacted chromatin state.
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影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
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DOI：
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发表时间：
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期刊：
Bioinformatics (Oxford, England)
影响因子：
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作者：
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通讯作者：
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64.5
作者：
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通讯作者：
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