Knockout of a papain-like cysteine protease gene OCP enhances blast resistance in rice.
Knockout of a papain-like cysteine protease gene OCP enhances blast resistance in rice.
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敲除木瓜蛋白酶样半胱氨酸蛋白酶 OCP 可增强水稻的稻瘟病抗性
DOI：
10.3389/fpls.2022.1065253
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
木瓜蛋白酶样半胱氨酸蛋白酶（PLCPs）在植物的免疫应答中起着重要的作用。在拟南芥中，已知几个同源基因参与防御病原体。然而，PLCPs对水稻疾病的影响在很大程度上是未知的。在这项研究中，我们表明PLCP，一种米谷蛋白α链前体（OCP），拟南芥蛋白酶RD 21的直系同源物（响应于脱水21），参与调节对稻瘟病的抗性，具有较短的损伤长度特征的敲除系（ocp-ko），通过CRISPR/Cas9技术产生。OCP在水稻各组织中均有表达，主要定位于细胞质中。我们证明了具有半胱氨酸蛋白酶活性的OCP与OsRACK 1A（receptor for activated C kinase 1）和OsSNAP 32（synaptosome-associated protein of 32 kD）在体内外物理相互作用，它们共定位于水稻细胞质中，但不能形成三元复合物。ocp-ko 1株系中富集了许多与植物免疫相关的基因，其表达量发生了显著变化。在ocp-ko 1中，茉莉酸（JA）和乙烯（ET）生物合成及调控基因表达上调，生长素外排转运蛋白表达下调。因此，OCP通过与OsRACK 1A或OsSNAP 32相互作用，影响许多抗性相关基因的表达谱，负调控水稻的稻瘟病抗性。此外，OCP可能通过抑制JA和ET信号通路的激活以及促进生长素信号通路而成为抗稻瘟病的基石。本研究为水稻抗病原菌提供了一个全面的PLCPs资源，也具有潜在的育种价值。
Papain-like cysteine proteases (PLCPs) play an important role in the immune response of plants. In Arabidopsis, several homologous genes are known to be involved in defending against pathogens. However, the effects of PLCPs on diseases that afflict rice are largely unknown. In this study, we show that a PLCP, an oryzain alpha chain precursor (OCP), the ortholog of the Arabidopsis protease RD21 (responsive to dehydration 21), participates in regulating resistance to blast disease with a shorter lesion length characterizing the knockout lines (ocp-ko), generated via CRISPR/Cas9 technology. OCP was expressed in all rice tissues and mainly located in the cytoplasm. We prove that OCP, featuring cysteine protease activity, interacts with OsRACK1A (receptor for activated C kinase 1) and OsSNAP32 (synaptosome-associated protein of 32 kD) physically in vitro and in vivo, and they co-locate in the rice cytoplasm but cannot form a ternary complex. Many genes related to plant immunity were enriched in the ocp-ko1 line whose expression levels changed significantly. The expression of jasmonic acid (JA) and ethylene (ET) biosynthesis and regulatory genes were up-regulated, while that of auxin efflux transporters was down-regulated in ocp-ko1. Therefore, OCP negatively regulates blast resistance in rice by interacting with OsRACK1A or OsSNAP32 and influencing the expression profiles of many resistance-related genes. Moreover, OCP might be the cornerstone of blast resistance by suppressing the activation of JA and ET signaling pathways as well as promoting auxin signaling pathways. Our research provides a comprehensive resource of PLCPs for rice plants in defense against pathogens that is also of potential breeding value.
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影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
影响因子：
11.6
作者：
Nakashima, Ayako;Chen, Letian;Shimamoto, Ko
通讯作者：
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DOI：
10.1186/s12284-021-00494-9
发表时间：
2021-06-05
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
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通讯作者：
Liu B
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影响因子：
6.9
作者：
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通讯作者：
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影响因子：
7.4
作者：
Kaschani, Farnusch;Shabab, Mohammed;van der Hoorn, Renier A. L.
通讯作者：
van der Hoorn, Renier A. L.