The OsOXO2, OsOXO3 and OsOXO4 Positively Regulate Panicle Blast Resistance in Rice.
The OsOXO2, OsOXO3 and OsOXO4 Positively Regulate Panicle Blast Resistance in Rice.
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Osoxo2，Osoxo3和Osoxo4在水稻中积极调节圆锥花序抗性。
DOI：
10.1186/s12284-021-00494-9
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发表时间：
2021-06-05
期刊：
影响因子：
--
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Dong J;Zhou L;Feng A;Zhang S;Fu H;Chen L;Zhao J;Yang T;Yang W;Ma Y;Wang J;Zhu X;Liu Q;Liu B
虽然穗瘟比叶瘟对水稻产量损失的破坏性更大，但克隆的穗瘟抗性基因仍然非常有限，并且穗瘟抗性的分子机制仍然很不清楚。本研究证实了抗稻瘟病品种BC 10中的三个草酸氧化酶基因OsOXO 2、OsOXO 3和OsOXO 4在水稻穗瘟抗性中发挥作用。OsOXO 2、OsOXO 3和OsOXO 4的表达通过穗瘟诱导。亚细胞定位分析表明，三种OXO蛋白均定位于细胞核和细胞质中。OsOXO 2、OsOXO 3和OsOXO 4同时沉默降低了水稻对穗瘟的抗性，而OsOXO 2、OsOXO 3和OsOXO 4过表达的水稻植株分别表现出增强的穗瘟抗性。在过表达植株中观察到更多的H2 O2和更高的PR基因的表达水平比在对照植株中，而沉默植株表现出更少的H2 O2和更低的PR基因的表达水平相比，对照植株。激素处理和激素信号相关基因表达分析表明，OXO基因介导的穗瘟抗性与JA和阿坝信号通路的激活有关，而与SA信号通路的抑制有关。OsOXO 2、OsOXO 3和OsOXO 4正调控水稻穗瘟抗性。OXO基因可以调节植物体内H2 O2的积累和PR基因的表达水平。此外，OXO基因介导的穗瘟抗性受阿坝、SA和JA的调控，可能与JA和阿坝信号通路的激活而SA信号通路的抑制有关。在线版本包含补充材料，可通过10.1186/s12284-021-00494-9获得。
Although panicle blast is more destructive to yield loss than leaf blast in rice, the cloned genes that function in panicle blast resistance are still very limited and the molecular mechanisms underlying panicle blast resistance remain largely unknown. In the present study, we have confirmed that the three Oxalate oxidase (OXO) genes, OsOXO2, OsOXO3 and OsOXO4 from a blast-resistant cultivar BC10 function in panicle blast resistance in rice. The expression of OsOXO2, OsOXO3 and OsOXO4 were induced by panicle blast inoculation. Subcellular localization analysis revealed that the three OXO proteins are all localized in the nucleus and cytoplasm. Simultaneous silencing of OsOXO2, OsOXO3 and OsOXO4 decreased rice resistance to panicle blast, whereas the OsOXO2, OsOXO3 and OsOXO4 overexpression rice plants individually showed enhanced panicle blast resistance. More H2O2 and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants, while the silencing plants exhibited less H2O2 and lower expression levels of PR genes compared to the control plants. Moreover, phytohormone treatment and the phytohormone signaling related gene expression analysis showed that panicle blast resistance mediated by the three OXO genes was associated with the activation of JA and ABA signaling pathways but suppression of SA signaling pathway. OsOXO2, OsOXO3 and OsOXO4 positively regulate panicle blast resistance in rice. The OXO genes could modulate the accumulation of H2O2 and expression levels of PR gene in plants. Moreover, the OXO genes mediated panicle blast resistance could be regulated by ABA, SA and JA, and may be associated with the activation of JA and ABA signaling pathways but suppression of the SA signaling pathway. The online version contains supplementary material available at 10.1186/s12284-021-00494-9.
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影响因子：
5.1
作者：
Carter, C;Graham, RA;Thornburg, RW
通讯作者：
Thornburg, RW
影响因子：
4.9
作者：
Molla, Kutubuddin A.;Karmakar, Subhasis;Datta, Karabi
通讯作者：
Datta, Karabi
影响因子：
5.5
作者：
Carrillo, Maria Gay C.;Goodwin, Paul H.;Cruz, Casiana M. Vera
通讯作者：
Cruz, Casiana M. Vera
影响因子：
4.3
作者：
Karmakar, Subhasis;Molla, Kutubuddin Ali;Datta, Karabi
通讯作者：
Datta, Karabi
影响因子：
6.5
作者：
Liu, Hang;Zhang, Yun-Hong;Du, Yu-Guang
通讯作者：
Du, Yu-Guang