Genetic control and intersite influences on the immune response to sperm whale myoglobin.
Genetic control and intersite influences on the immune response to sperm whale myoglobin.
复制标题
遗传控制和位点间对抹香鲸肌红蛋白免疫反应的影响。
DOI：
10.1007/978-1-4684-4331-8_6
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发表时间：
1982
影响因子：
--
通讯作者：
Atassi,MZ
中科院分区：
文献类型：
--
作者：
David,CS;Atassi,MZ
对精子-鲸鱼肌红蛋白(Mb)的精确抗原结构的确定使我们能够将注意力集中在控制和调节对蛋白质抗原的免疫反应的分子和细胞因素上。我们的研究表明，对抹香鲸Mb的免疫反应是由小鼠主要组织相容性复合体(H-2)I区的基因控制的。更重要的是，对合成抗原点的反应都受到不同的基因控制。抗体对抗原部位的识别与免疫物种和初次免疫后获得抗血清的时间无关(从9天到一年)。兔、山羊、猪、猫、鸡以及近交系和近交系小鼠的抗血清也能识别相同的位点。小鼠B细胞识别的相同位置也被小鼠T细胞识别。没有观察到抗体亲和力的有意义的遗传控制。用自身Mb免疫动物很容易产生自身免疫抗体和T淋巴细胞增殖反应。对于小鼠Mb，自身免疫T淋巴细胞反应受遗传控制，并与H-2基因复合体的I-A和H-2D末端定位。在最近的其他研究中，我们使用几个Mb变体表明，抗原位点的结合能力完全由抗原位点(实际接触残基)和靠近该位点的残基(即环境残留物)中的取代所解释。对Mb的整体反应受站点间影响的调节，这种影响可以是合作(帮助)性质的，也可以是抑制性质的。最后，对蛋白质上个别抗原部位的反应的遗传控制不仅取决于宿主的遗传构成，还取决于个别部位的化学性质。映射对给定抗原位点的反应的H-2亚区也可以识别以前在同源蛋白质中无法识别的其他位点，如果这些位点的化学性质被适当改变的话。
Determination of the precise antigenic structure of sperm-whale myoglobin (Mb) has enabled us to focus our attention on the molecular and cellular factors that control and regulate the immune responses to the protein antigen. Our studies have shown that the immune responses to sperm-whale Mb are controlled by genes in the I region of the major histocompatibility complex (H-2) of mice. More importantly, the responses to the synthetic antigenic sites are each under separate genetic control. The recognition of the antigenic sites by antibodies is independent of the immunized species and of the time the antisera are obtained after the initial immunization (from nine days up to a year). The same sites are recognized by antisera raised in rabbit, goat, pig, cat, chicken and outbred and inbred mice. The same sites recognized by mouse B-cells are also recognized by mouse T-cells. No meaningful genetic control of antibody affinity was observed. Autoimmune antibody and T-lymphocyte proliferative responses were readily generated by immunizing an animal with self-Mb. With mouse Mb, the autoimmune T-lymphocyte response was under genetic control and mapped with the I-A and the H-2D end of the H-2 gene complex. In other recent studies we have shown, using several Mb variants, that the binding capacity of an antigenic site is fully accounted for by substitutions in the antigenic sites (actual contact residues) and in residues close (within 7.Å) to the sites (i.e. environmental residues). The overall response to Mb is regulated by inter-site influences which can either be of a cooperative (help) nature or of a suppressive nature. Finally, genetic control of the responses to individual antigenic sites on a protein is not only determined by the genetic constitution of the host but also by the chemical properties of the individual sites. The H-2 subregions mapping the responses to given antigenic sites can also recognize other sites, which were previously unrecognizable in a homologous protein, if the chemical properties of these sites are suitably altered.
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DOI：
--
发表时间：
1981
期刊：
Immunological Communications
影响因子：
--
作者：
C. Young;O'Connor Gp;Atassi Mz
通讯作者：
Atassi Mz
DOI：
--
发表时间：
1981
期刊：
Immunological Communications
影响因子：
--
作者：
M. Atassi;Sally S. Twining;Hermann Lehmann;C. David
通讯作者：
C. David
影响因子：
4.1
作者：
M. Atassi;A. Kazim
通讯作者：
A. Kazim
抹香鲸肌红蛋白的高碘酸盐氧化以及蛋氨酸残基在抗原抗体反应中的作用。
影响因子：
4.1
作者：
M. Atassi
通讯作者：
M. Atassi
通过兔肌球蛋白免疫产生自身抗体。
DOI：
--
发表时间：
1978
期刊：
Immunochemistry
影响因子：
--
作者：
A. Kazim;M. Atassi
通讯作者：
M. Atassi