Comparison of in vitro and in vivo activities associated with the G6PD allozyme polymorphism in Drosophila melanogaster.
Comparison of in vitro and in vivo activities associated with the G6PD allozyme polymorphism in Drosophila melanogaster.
复制标题
与果蝇 G6PD 同种酶多态性相关的体外和体内活性比较。
作者：
W. Eanes;L. Katona;M. Longtine
Earlier studies of the A and B allozymes at the G6pd locus show a differential ability of the genotypes to suppress the loss of viability associated with a low activity 6-phosphogluconate dehydrogenase mutation, 6Pgdlo1. This observation indicates a relatively lower activity for the A allozyme genotype, but it is not known if this level of suppression required a large difference in in vivo activity. To clarify this difference an analysis of the biochemical properties of the purified allozymes was carried out, as well as an analysis of the activity level associated with an original low activity P element-derived allele which had partially reverted and lost its suppression ability. G6PD activity and protein level were studied in 47 X chromosome lines from North America. The A genotype averages a 9% lower Vmax. From analysis of the correlation between G6PD activity and protein level it remains unclear whether the allozyme Vmax difference results from dissimilarity in protein level or kcat. At 25 degrees and physiological pH, comparative studies of the steady-state kinetics show the two purified allozyme variants differ significantly in their KM values for glucose-6-phosphate and NADP, and the K1 for NADPH. In aggregate these parameters predict the A genotype possesses a 20% lower in vitro catalytic efficiency. A partial revertant of a P element-derived low activity B variant, was shown to lose the ability to suppress 6Pgdlo1 low viability after acquiring only 60% of normal B activity. This last comparison shows the A genotype activity must be reduced in vivo by at least 40%.
影响因子：
10.7
作者：
White,MM;Mane,SD;Richmond,RC
通讯作者：
Richmond,RC
果蝇活力相互作用、体内活性和 G6PD 多态性。
影响因子：
3.3
作者：
Eanes,WF
通讯作者：
Eanes,WF
果蝇的遗传不稳定性：基因转换引起的 P 元件诱变。
DOI：
10.1073/pnas.85.17.6455
发表时间：
1988
影响因子：
11.1
作者：
Geyer,PK;Richardson,KL;Corces,VG;Green,MM
通讯作者：
Green,MM