Continuous wave irradiation at 0.1 terahertz facilitates transmembrane transport of small molecules.
Continuous wave irradiation at 0.1 terahertz facilitates transmembrane transport of small molecules.
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DOI：
10.1016/j.isci.2022.103966
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发表时间：
2022-03-18
期刊：
影响因子：
5.8
通讯作者：
Lu X
中科院分区：
文献类型：
--
作者：
Hu E;Zhang Q;Shang S;Jiang Y;Lu X
由于太赫兹辐射对生物分子间相互作用的影响，其生物效应越来越受到人们的关注。本研究旨在研究太赫兹辐射对细胞膜的影响，特别是细胞膜通透性的影响。我们发现，0.1太赫兹辐射促进FM 4 -64标记的细胞的内吞作用和动力蛋白的抑制减弱，但没有完全取消太赫兹促进的内吞作用。此外，0.1太赫兹辐射也促进了跨膜的罗丹明，以及化合物GDC 0941和H89，证明了通过共聚焦显微镜观察和蛋白质印迹分析，分别。这些发现表明0.1THz辐射通过促进细胞内吞和扩散过程来促进小分子的跨膜转运。我们的研究为THz影响细胞膜通透性提供了直接证据，拓宽了THz对细胞生理过程的影响，并暗示了THz在调节细胞膜功能方面的潜在应用。THz辐射增强神经元细胞的内吞活性THz辐射增加罗丹明衍生物向细胞的渗透THz辐射促进小分子药物GDC 0941和H89的细胞摄取转运现象;辐射;膜
The bioeffects of terahertz (THz) radiation received growing attention because of its influence on the interactions between biomolecules. Our work aimed to investigate the effects of THz irradiation on cell membrane, especially cell membrane permeability. We found that 0.1 THz irradiation promoted the endocytosis of FM4-64-labeled cells and the inhibition of dynamin attenuated but did not fully abolish the THz promoted endocytosis. Moreover, 0.1 THz irradiation also promoted the transmembrane of the rhodamine, as well as the chemical compounds GDC0941 and H89, evidenced by the confocal microscope observation and the western blotting analysis, respectively. These findings demonstrated 0.1 THz irradiation facilitated the transmembrane transport of small molecules by promoting both the cellular endocytosis and the diffusion process. Our study provided direct evidence that THz could affect the cell membrane permeability, broadened the THz affected cellular physiological processes, and implied its potential application in regulating the cell membrane functions. THz irradiation enhances endocytic activity of neuronal cells THz irradiation increases the permeation of rhodamine derivatives into cells THz irradiation promotes the cellular uptake of small drugs GDC0941 and H89 Transport phenomena; Radiation; Membranes
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影响因子：
11.8
作者：
Macia, Eric;Ehrlich, Marcelo;Kirchhausen, Tomas
通讯作者：
Kirchhausen, Tomas
影响因子：
5.3
作者：
Jiang, Min;Chen, Gong
通讯作者：
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影响因子：
3.4
作者：
Abufadda, Mahmoudh;Erdelyi, Anita;Molnar, Laszlo
通讯作者：
Molnar, Laszlo
DOI：
10.35248/2157-7439.19.10.535
发表时间：
2019-01-01
期刊：
Journal of nanomedicine & nanotechnology
影响因子：
--
作者：
Ghann, William;Kang, Hyeonggon;Uddin, Jamal
通讯作者：
Uddin, Jamal
影响因子：
3.4
作者：
Borovkova, Mariia;Khodzitsky, Mikhail;Meglinski, Igor
通讯作者：
Meglinski, Igor