Single-cell RNA-sequencing of migratory breast cancer cells: discovering genes associated with cancer metastasis.
Single-cell RNA-sequencing of migratory breast cancer cells: discovering genes associated with cancer metastasis.
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DOI：
10.1039/c9an01358j
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发表时间：
2019-12-02
期刊：
影响因子：
--
通讯作者：
Yoon E
中科院分区：
文献类型：
--
作者：
Chen YC ;Sahoo S ;Brien R ;Jung S ;Humphries B ;Lee W ;Cheng YH ;Zhang Z ;Luker KE ;Wicha MS ;Luker GD ;Yoon E
大量证据表明，乳腺癌转移发生于上皮-间质转化（EMT）细胞和癌症干细胞样细胞（CSCs）。我们利用微流控装置富集迁移乳腺癌细胞，增强其肿瘤形成和转移能力，通过高通量单细胞rna测序鉴定迁移细胞中差异表达的基因。迁移细胞表现出EMT和CSCs的整体特征，标记基因的表达变化，并随着时间的推移保持EMT的表达谱。通过单细胞分辨率，我们发现了中间的EMT状态和不同的上皮和间充质迁移细胞亚群，这表明乳腺癌细胞可以在保持上皮状态的同时快速迁移。迁移细胞在氧化应激、线粒体形态和蛋白酶体的调节方面表现出不同的特征，揭示了药物的潜在脆弱性和意想不到的后果。我们还发现了与乳腺癌细胞迁移和预后相关的新基因，作为潜在的预后生物标志物和治疗靶点，以阻止转移中的迁移细胞。迁移乳腺癌细胞的单细胞rna测序：发现与癌症转移相关的基因
Considerable evidence suggests breast cancer metastasis arises from cells undergoing epithelial-to-mesenchymal-transition (EMT) and cancer stem-like cells (CSCs). Using a microfluidic device that enriches migratory breast cancer cells with enhanced capacity for tumor formation and metastasis, we identified genes differentially expressed in migratory cells by high-throughput single-cell RNA-sequencing. Migratory cells exhibited overall signatures of EMT and CSCs with variable expression of marker genes, and they retained expression profiles of EMT over time. With single-cell resolution, we discovered intermediate EMT states and distinct epithelial and mesenchymal sub-populations of migratory cells, indicating breast cancer cells can migrate rapidly while retaining an epithelial state. Migratory cells showed differential profiles for regulators of oxidative stress, mitochondrial morphology, and the proteasome, revealing potential vulnerabilities and unexpected consequences of drugs. We also identified novel genes correlated with cell migration and outcomes in breast cancer as potential prognostic biomarkers and therapeutic targets to block migratory cells in metastasis. Single-cell RNA-sequencing of migratory breast cancer cells: discovering genes associated with cancer metastasis
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EMT亚型影响上皮可塑性和细胞迁移模式。
影响因子：
11.8
作者：
Aiello NM;Maddipati R;Norgard RJ;Balli D;Li J;Yuan S;Yamazoe T;Black T;Sahmoud A;Furth EE;Bar-Sagi D;Stanger BZ
通讯作者：
Stanger BZ
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
10.9
作者：
Li Z
通讯作者：
Li Z
影响因子：
5.9
作者：
Liu S;Cong Y;Wang D;Sun Y;Deng L;Liu Y;Martin-Trevino R;Shang L;McDermott SP;Landis MD;Hong S;Adams A;D'Angelo R;Ginestier C;Charafe-Jauffret E;Clouthier SG;Birnbaum D;Wong ST;Zhan M;Chang JC;Wicha MS
通讯作者：
Wicha MS
DOI：
10.1097/pai.0b013e31821a0be9
发表时间：
2011-12-01
影响因子：
1.6
作者：
Maciejczyk, Adam;Szelachowska, Jolanta;Surowiak, Pawel
通讯作者：
Surowiak, Pawel