Nanobody: the "magic bullet" for molecular imaging?
Nanobody: the "magic bullet" for molecular imaging?
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作者：
Chakravarty R;Goel S;Cai W
分子成像涉及在细胞和分子水平上对体内生物过程的非侵入性研究，在更好地理解和治疗各种疾病方面可以发挥不同的作用。最近，被称为“纳米抗体”的单域抗原结合片段经过生物工程改造并测试用于分子成像应用。纳米抗体的小分子尺寸（~15 kDa）和互补决定区（CDR）的适当配置提供了许多适合成像应用的理想特性，例如快速靶向和快速血液清除、高溶解度、高稳定性、易于克隆、模块化性质以及与空腔和难以接近的抗原结合的能力。使用基于纳米体的探针，多种成像技术（例如基于放射性核素的成像技术、光学成像技术和超声成像技术）已被用于各种疾病模型中靶标表达的可视化。本综述总结了基于纳米抗体的探针在分子成像应用中的最新进展。迄今为止报道的临床前数据非常有前景，预计基于纳米抗体的分子成像剂将在各种疾病的诊断和管理中发挥重要作用。
Molecular imaging involves the non-invasive investigation of biological processes in vivo at the cellular and molecular level, which can play diverse roles in better understanding and treatment of various diseases. Recently, single domain antigen-binding fragments known as 'nanobodies' were bioengineered and tested for molecular imaging applications. Small molecular size (~15 kDa) and suitable configuration of the complementarity determining regions (CDRs) of nanobodies offer many desirable features suitable for imaging applications, such as rapid targeting and fast blood clearance, high solubility, high stability, easy cloning, modular nature, and the capability of binding to cavities and difficult-to-access antigens. Using nanobody-based probes, several imaging techniques such as radionuclide-based, optical and ultrasound have been employed for visualization of target expression in various disease models. This review summarizes the recent developments in the use of nanobody-based probes for molecular imaging applications. The preclinical data reported to date are quite promising, and it is expected that nanobody-based molecular imaging agents will play an important role in the diagnosis and management of various diseases.
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影响因子：
6.7
作者：
Choi KY;Liu G;Lee S;Chen X
通讯作者：
Chen X
影响因子：
16.6
作者：
Chen, Feng;Ellison, Paul A.;Lewis, Christina M.;Hong, Hao;Zhang, Yin;Shi, Sixiang;Hernandez, Reinier;Meyerand, M. Elizabeth;Barnhart, Todd E.;Cai, Weibo
通讯作者：
Cai, Weibo
影响因子：
--
作者：
Gainkam, Lea Olive Tchouate;Caveliers, Vicky;Lahoutte, Tony
通讯作者：
Lahoutte, Tony
影响因子：
12.4
作者：
Baum RP;Kulkarni HR
通讯作者：
Kulkarni HR
DOI：
10.1073/pnas.88.17.7859
发表时间：
1991-09-01
影响因子：
11.1
作者：
CYBULSKY, MI;FRIES, JWU;COLLINS, T
通讯作者：
COLLINS, T