Collection and culture of alveolar bone marrow multipotent mesenchymal stromal cells from older individuals

Collection and culture of alveolar bone marrow multipotent mesenchymal stromal cells from older individuals
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老年人肺泡骨髓多能间充质基质细胞的采集和培养

DOI:
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发表时间:
2009
影响因子:
4
通讯作者:
Y. Ogata
Y. Ogata
中科院分区:
生物学2区
文献类型:
--
作者:
Juan Han;H. Okada;H. Takai;Youhei Nakayama;T. Maeda;Y. Ogata

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In this work, we examined the culture condition of alveolar bone marrow multipotent mesenchymal stromal cells (ABMMSCs), aiming to apply regenerative therapy to older periodontitis patients. To better understand the character of cultured cells from alveolar bone marrow, the expression profiles of well‐known genes and their responses to the induction of osteogenic, chondrogenic, or adipogenic differentiation were examined. Using αMEM‐based culture, ABMMSCs could be obtained from older individuals than in previous reports. Interestingly, ABMMSCs expressing Klf4 were able to differentiate into osteoblasts. The prediction of differentiation potential by Klf4 could be a useful guide for further improvement of the culture conditions required to culture ABMMSCs derived from older individuals. J. Cell. Biochem. 107: 1198–1204, 2009. © 2009 Wiley‐Liss, Inc.
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