Impaired neutrophil chemotaxis in Crohn's disease relates to reduced production of chemokines and can be augmented by granulocyte-colony stimulating factor.
Impaired neutrophil chemotaxis in Crohn's disease relates to reduced production of chemokines and can be augmented by granulocyte-colony stimulating factor.
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DOI：
10.1111/j.1365-2036.2006.03016.x
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发表时间：
2006-08-15
影响因子：
7.6
通讯作者：
Segal AW
中科院分区：
文献类型：
--
作者：
Harbord MW;Marks DJ;Forbes A;Bloom SL;Day RM;Segal AW
中性粒细胞募集缺陷已被描述为克罗恩病的原发性致病性异常。斑蝥素诱导的水泡提供了一种新的研究工具来评估急性炎症期间的细胞内流和炎症介质的产生，并允许测量治疗对这些参数的影响。确定克罗恩病中性粒细胞组织渗透减少是否与炎症介质的产生受损有关，以及是否可以通过粒细胞集落刺激因子（G-CSF）逆转。在斑蝥素水疱24小时内测量中性粒细胞、单核细胞/巨噬细胞数量和炎症介质。用水疱液作为化学引诱剂，体外评估中性粒细胞趋化性。测定s.c.g - csf对水疱表型的影响。在克罗恩病患者中，迁移到水疱中的中性粒细胞明显减少。中性粒细胞趋化因子的产生减少，但其他炎症介质没有减少。这与体外趋化性降低显著相关。差异与caspase-募集结构域15基因型无关。G-CSF显著增加对照组和克罗恩病患者的水疱中性粒细胞浓度。克罗恩病急性炎症期间中性粒细胞迁移减少与适当的化学引诱剂的产生受损有关。G-CSF治疗增加中性粒细胞组织迁移，这可能部分解释了其观察到的治疗效果。
Defective neutrophil recruitment has been described as a primary pathogenic abnormality in Crohn’s disease. Cantharidin-induced blisters provide a novel investigative tool to assess cellular influx and inflammatory mediator production during acute inflammation and allows the effects of therapy on these parameters to be measured. To determine whether reduced neutrophil tissue penetration in Crohn’s disease relates to impaired production of inflammatory mediators, and whether it can be reversed by granulocyte-colony stimulating factor (G-CSF). Neutrophil and monocyte/macrophage populations and inflammatory mediators were measured in cantharidin blisters at 24 h. Neutrophil chemotaxis was assessed in vitro using blister fluid as the chemoattractant. The effect of s.c. G-CSF on blister phenotype was determined. Significantly fewer neutrophils migrated into blisters in Crohn’s patients. The production of neutrophil chemokines, but not other inflammatory mediators, was reduced. This significantly correlated with reduced chemotaxis in vitro. Differences were unrelated to caspase-recruitment domain 15 genotype. G-CSF significantly increased blister neutrophil concentrations in control subjects and Crohn’s patients. Reduced neutrophil migration during acute inflammation in Crohn’s disease is associated with impaired production of appropriate chemoattractants. G-CSF therapy increases neutrophil tissue migration, which may partially account for its observed therapeutic effect.
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影响因子：
3.4
作者：
Brunner, M;Schmiedberger, A;Müller, M
通讯作者：
Müller, M
影响因子：
3.2
作者：
Matsumoto, T;Nakamura, S;Nagura, H
通讯作者：
Nagura, H
DOI：
10.1016/j.bpg.2003.12.005
发表时间：
2004-06-01
影响因子：
3.2
作者：
Ekbom, A
通讯作者：
Ekbom, A
影响因子：
5.4
作者：
Gijsbers, K;Van Assche, G;Van Damme, J
通讯作者：
Van Damme, J
影响因子：
3.1
作者：
Korzenik, JR;Dieckgraefe, BK
通讯作者：
Dieckgraefe, BK